文献类型：期刊文献

中文题名：Enhanced bioproduction of chitin in engineered Pichia pastoris

作者：Xue Zhang[1];Chunyue Zhang[1,2];Mian Zhou[1];Quanming Xia[1,2];Liqiang Fan[1,2];Liming Zhao[1,2]

机构：[1]State Key Laboratory of Bioreactor Engineering,School of Biotechnology,East China University of Science and Technology,Shanghai,200237,China;[2]Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCICBT),Shanghai,200237,China

年份：2022

卷号：47

期号：3

起止页码：1051

中文期刊名：Food Bioscience

外文期刊名：食品生物科学（英文）

收录：Scopus;PubMed

基金：supported by the National Key R&D Program of China(2019YFD0901805);the 111 Project(B18022);the Fundamental Research Funds for the Central Universities(22221818014).

语种：英文

中文关键词：Chitin;Pichia pastoris;Metabolic engineering

摘要：Biopolymer chitin and its derivatives have a wide range of applications in different fields.Chitin is primarily produced from marine crustacean by strong acid and strong alkali treatment.To reduce the environmental hazardous in the production of chitin,post-fermentation fungal biomass waste like fungi cell walls provides a viable alternative source for chitin.In this study,improved chitin content in yeast cell wall was achieved by overexpressing chitin biosyntheic pathway related genes.The chitin content was 51.5μg/mg cell wall and 122.9μg/mg cell wall in strain GS-1.10(gfat,uap and chs1 genes overexpression)and strain GS-2.6(gfat,gna1,agm1,uap and chs1 genes overexpression),which were 44.3%and 244.4%higher than that in control strain GS115,respectively.By overexpressing another chitin synthase gene(chs3),the chitin content in strain GS-3.10 was further increased to 136.2μg/mg cell wall,which was 10.8%and 281.6%higher than that in GS-2.6 and in control GS115,respectively.Moreover,chitin yield was further improved by various culture conditions optimization,and reached 162.4μg/mg cell wall,which was 4.43 times of that in the starting strain GS115.The final titer of chitin was 2.23 g/L culture broth in 84 h fed-batch fermentation in a 5 L bioreactor.To our knowledge,this is the first report of chitin production in engineered Pichia pastoris via biosynthetic pathway enhancement.