文献类型：期刊文献

中文题名：乳酸链球菌素Z纯化脱色小试工艺研究

英文题名：SMALL SCALE RESEARCH ON PURIFICATION AND DECOLORING PROCESS OF NISIN Z

作者：刘立毅[1];宁方红[1,2];徐环昕[1,2];江邦和[1,2];刘坐镇[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]上海华震科技有限公司,上海200237

年份：2014

卷号：30

期号：5

起止页码：429

中文期刊名：离子交换与吸附

外文期刊名：Ion Exchange and Adsorption

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

语种：中文

中文关键词：吸附树脂;乳酸链球菌素Z;双水相;制备液相色谱

外文关键词：Adsorption resin;;Nisin Z;;Aqueous two-phase;;Preparative liquid chromatography

摘要：Nisin Z的工业生产中利用细胞菌体吸附Nisin Z,离心后的发酵上清液(Nisin Z效价1000IU/m L左右)则通常被当成废液处理。本文建立大孔吸附树脂富集浓缩,丙酮-盐双水相脱色及中压制备型高效液相色谱精制三步法,从低浓度的发酵液上清液中回收较高纯度Nisin Z的方法。经过HZM-3树脂吸附和解吸,乳链菌肽Z的纯度由0.28%提高了95倍至26.65%,双水相脱色后Nisin Z纯度进一步提高至55.43%,经过制备分离后Nisin Z纯度达到94.12%。总回收率为71.39%,脱色率为95.7%。
The Nisin Z fermentation supernatant with an antibiotic activity of about 1000IU/m L removed by centrifugal sedimentation was treated as waste water after Nisin Z being absorbed to the production strain cells in industrial production. A protocol to obtain high purity Nisin Z by a combination of macroporous adsorption resin chromatography, acetone-salt aqueous two-phase and medium pressure preparative liquid chromatography from fermentation supernatant was established. The purity of Nisin Z increased about 95 fold from 0.28% to 26.65% after enriching with HZM-3 resin and increased to 55.43% followed by aqueous two-phase decolorization. With separation of the preparative liquid chromatography, the purity of Nisin Z was 94.12%. The overall recovery and decolorization efficiency were 71.39% and 95.7%, respectively.