文献类型：期刊文献

英文题名：BMP-2/CPC scaffold with dexamethasone-loaded blood clot embedment accelerates clinical bone regeneration

作者：Liu, Yutong[1,2];Lin, Dan[1,2];Hong, Hua[1,2];Jiang, Chuan[4];Yuan, Yuan[1,2];Wang, Jinwu;Hu, Ruyin[3];Li, Bo[3,3];Liu, Changsheng[1,2]

机构：[1]East China Univ Sci & Technol, Engn Res Ctr Biomat, Minist Educ, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Guizhou Prov Peoples Hosp, Dept Orthopaed, Guiyang 550002, Peoples R China;[4]Shanghai Jiao Tong Univ, Dept Orthopaed Surg, Peoples Hosp 9, Shanghai 200011, Peoples R China

年份：2022

卷号：14

期号：5

起止页码：2874

外文期刊名：AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000809451000003)】；

基金：The authors wish to express their gratitude to the financial supports from the National Natural Science Foundation of China for Innovative Research Groups (No. 51621002), and Nation-al Natural Science Foundation of China (No. 31771040, No. 31971264).

语种：英文

外文关键词：BMP-2; calcium phosphate cement; dexamethasone; blood clot; clinical bone regeneration

摘要：Delayed repair caused by prolonged inflammatory response might lead to clinical nonunion and failed bone regeneration. The design of desirable biomaterials requires precise regulation of the initial osteoimmune responses and efficient osteoinductive capacity to facilitate later bone regeneration. Herein, a Dex-loaded blood clot-embedded BMP-2/CPC scaffold (Dex/blood/BMP-2/CPC) was fabricated for clinical bone regeneration with the sequential release of dexamethasone (Dex), a clinical immunosuppression drug, and BMP-2, a potent osteogenic growth factor. The introduction of Dex at a BMP-2/Dex ratio of 1/6 effectively facilitated M2 polarization of macrophages and exerted a synergetic effect on BMP-2-mediated osteogenic differentiation. The highest in vivo bone regenerative efficacy was achieved by Dex/blood/BMP-2/CPC scaffold with a 1/6 BMP-2/Dex dose, exhibiting significantly enhanced endochondral ossification and attenuated bone resorption in an ectopic model, as well as reduced fibrosis at the orthotopic defect site. Blood clot embedment further provides nutrition and cytokines for the endochondral ossification process. On these bases, a pilot clinical trial was carried out and the Dex/blood/BMP-2/CPC scaffold was demonstrated to accelerate fracture healing, improve therapeutic quality, and eliminate local inflammation compared to current bone regenerative treatment. Taken together, this work designed an immunoregulatory and osteoinductive Dex/blood/BMP-2/CPC scaffold, which might provide new insights for future biomaterial development (Trial registration: ChiCTR2100047693).