文献类型：期刊文献

英文题名：Ecotoxicological evaluation of low-concentration bisphenol A exposure on the soil nematode Caenorhabditis elegans and intrinsic mechanisms of stress response in vivo

作者：Zhou, Dong[1];Yang, Jie[2];Li, Hui[1];Lu, Qiang[1];Liu, Yong-di[1];Lin, Kuang-fei[1]

机构：[1]East China Univ Sci & Technol, State Environm Protect Key Lab Environm Risk Asse, State Key Lab Bioreactor Engn, Sch Resources & Environm Engn, Shanghai, Peoples R China;[2]Shanghai Acad Environm Sci, Res Inst Wastes & Soil Remediat, Shanghai, Peoples R China

年份：2016

卷号：35

期号：8

起止页码：2041

外文期刊名：ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY

收录：;EI(收录号：20163002646528);WOS:【SCI-EXPANDED(收录号:WOS:000380057100018)】；

基金：The authors have no conflicts of interest. The wild-type nematode strain was provided by the Caenorhabditis Genetics Center, which is funded by the National Institutes of Health's Office of Research Infrastructure Programs (P40 OD010440). The present study was also financially supported by the National Environmental Protection Public Welfare Science and Technology Research Program of China (201309047), National Natural Science Foundation of China (41273109, 51378208, 51578240), the Program for New Century Excellent Talents in University (NCET-13-0797), the Fok Ying Tung Education Foundation (141077), the Innovation Program of Shanghai Municipal Education Commission (14ZZ059), the Innovation Program of Shanghai Municipal of Science and Technology Commission (15DZ1205804), and the Fundamental Research Funds for the Central Universities (222201313008, 222201516015).

语种：英文

外文关键词：Caenorhabditis elegans; Bisphenol A; Multiple toxicological endpoints; Cell apoptosis; Stress-related gene expression

摘要：As a representative species of nematodes, Caenorhabditis elegans is an attractive animal model for evaluating ecotoxicological effects and intrinsic mechanisms of the stress response in vivo. To acquire a better knowledge of environmental effects of bisphenol A (BPA), ecotoxicological evaluations were conducted using C. elegans on the physiological (growth, locomotion behaviors, and reproduction), biochemical (lipofuscin accumulation, reactive oxygen species production, and cell apoptosis), and molecular (stress-related gene expression) responses. Nematodes were exposed to BPA (0.001-10 mu M) in 2 assay systems (L4 larvae for 24h and L1 larvae for 72h). Exposure to BPA could significantly (p<0.05) alter body length, locomotion behaviors, brood size, cell apoptosis, and selected stress-related gene expression. At the physiological level, BPA exerted adverse effects on nematodes at the microgram per liter level in both assay systems, with head thrashes as the most sensitive endpoint. At the biochemical level, apoptosis degree showed increases at concentrations above 0.1 mu M in both assay systems. At the molecular level, BPA induced increases in selected stress-related gene expression, even at the lowest tested concentration. In addition, BPA-induced cell apoptosis was suggested as a potential mode of action, resulting in adverse physiological effects. Therefore, BPA exposure was speculated to impose developmental, reproductive, and neurobehavioral toxicities on C. elegans and caused variations of stress-related gene expression. Environ Toxicol Chem 2016;35:2041-2047. (c) 2016 SETAC