文献类型：期刊文献

英文题名：Differential gene expression of human CD34⁺ hematopoietic stem and progenitor cells before and after culture

作者：Li, QL; Cai, HB; Liu, QW; Tan, WS

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2006

卷号：28

期号：6

起止页码：389

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000236846300004)】；

语种：英文

外文关键词：cDNA array; CD34(+) hematopoietic stem cells; cord blood; ex vivo expansion; SMART-PCR

摘要：Ex vivo expanded CD34(+) hematopoietic stem and progenitor cells (HSPCs) have compromised homing and engraftment capacities. To investigate underlying mechanisms for functional changes of expanded HSPCs, we compared gene expression profiling of cultured and fresh CD34(+) cells derived from cord blood using SMART-PCR and cDNA array: 20 genes were up-regulated while 25 genes were down-regulated in cultured CD34(+) HSPCs. These differentially expressed genes are involved primarily in proliferation, differentiation, apoptosis, and homing.