文献类型：期刊文献

英文题名：Fabrication of three-dimensional poly(ε-caprolactone) scaffolds with hierarchical pore structures for tissue engineering

作者：Zhang, Qingchun[1];Luo, Houyong[2];Zhang, Yan[1];Zhou, Yan[2];Ye, Zhaoyang[2];Tan, Wensong[2];Lang, Meidong[1]

机构：[1]E China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Sch Bioengn, Shanghai 200237, Peoples R China

年份：2013

卷号：33

期号：4

起止页码：2094

外文期刊名：MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

收录：;EI(收录号：20131316143065);WOS:【SCI-EXPANDED(收录号:WOS:000317528700035)】；

基金：This research was supported by the Fundamental Research Funds for the Central Universities, the National Natural Science Foundation of China (20804015, 31000424), Specialized Research Fund for the Doctoral Program of Higher Education (200802511021, 20100074120009), Shanghai Pujiang Program (10PJ1402200), "Shu Guang" Project of Shanghai Municipal Education Commission, the Natural Science Foundation of Shanghai (08ZR1406000) and the Program for Changjiang Scholars and Innovative Research Team in University (IRT0825).

语种：英文

外文关键词：Poly(epsilon-caprolactone); Scaffolds; Pore size; Fibroblasts

摘要：The physical properties of tissue engineering scaffolds such as microstructures play important roles in controlling cellular behaviors and neotissue formation. Among them, the pore size stands out as a key determinant factor. In the present study, we aimed to fabricate porous scaffolds with pre-defined hierarchical pore sizes, followed by examining cell growth in these scaffolds. This hierarchical porous microstructure was implemented via integrating different pore-generating methodologies, including salt leaching and thermal induced phase separation (TIPS). Specifically, large (L, 200-300 mu m), medium (M, 40-50 mu m) and small (S, <10 mu m) pores were able to be generated. As such, three kinds of porous scaffolds with a similar porosity of similar to 90% creating pores of either two (LS or MS) or three (LMS) different sizes were successfully prepared. The number fractions of different pores in these scaffolds were determined to confirm the hierarchical organization of pores. It was found that the interconnectivity varied due to the different pore structures. Besides, these scaffolds demonstrated similar compressive moduli under dry and hydrated states. The adhesion, proliferation, and spatial distribution of human fibroblasts within the scaffolds during a 14-day culture were evaluated with MU assay and fluorescence microscopy. While all three scaffolds well supported the cell attachment and proliferation, the best cell spatial distribution inside scaffolds was achieved with LMS, implicating that such a controlled hierarchical microstructure would be advantageous in tissue engineering applications. (c) 2013 Elsevier B.V. All rights reserved.