文献类型：期刊文献

英文题名：Probing sugar-lectin recognitions in the near-infrared region using glyco-diketopyrrolopyrrole with aggregation-induced-emission

作者：Hang, Yandi[1];He, Xiao-Peng[1];Yang, Lin[1];Hua, Jianli[1]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat Inst Fine Chem, Shanghai 200237, Peoples R China

年份：2015

卷号：65

起止页码：420

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20144600207788);WOS:【SCI-EXPANDED(收录号:WOS:000348880000060)】；

基金：We thank the 973 Project (2013CB733700), the National Natural Science Foundation of China (21372082, 2116110444, 21172073, 21176076, 21202045), the Key Project of Shanghai Science and Technology Commission (13NM1400900) and the Fundamental Research Funds for Central Universities. J.-L. Hua appreciates Prof. H. Tian very much for his helpful discussion and valuable comments.

语种：英文

外文关键词：Sugar; Lectin; AIE; NIR; Fluorescence

摘要：This study describes the construction of aggregation-induced-emission (AIE)-based glycosyl probes for the sensitive and selective detection of sugar-lectin interactions in the near-infrared (NIR) region. Mannosyl and galactosyl diketopyrrolopyrrole (DPP) derivatives were effectively synthesized by the Cu (I)-catalyzed azide-alkyne 1.3-dipolar cycloaddition reaction. We observed that these glycodyes had typical AIE behaviors in a semi-aqueous solution with a strong fluorescence (FL) emission in the NIR region. In a buffer solution, the glycosyl DPPs at the quenching state showed sharply increased FL upon addition of a selective lectin that recognizes the glycosyl moiety of the compounds with nanomolar limits of detection. In contrast, addition of unselective lectins, proteins and ions did not fluctuate the FL Scanning electron microscopy analyses suggested that the FL generation could probably be a result of AIE of the glyco-DPPs upon complexation with lectins. These glyco-DPPs, to the best of our knowledge, represent the first fiuorogenic NE-based probes that can sense lectins in the NIR region, providing insights for the further extension towards low-background in vivo targeted imaging of tissues that express a lectin. (C) 2014 Elsevier B.V. All rights reserved.