文献类型：期刊文献

中文题名：甲醇流加策略对重组毕赤酵母表达人肝再生增强因子的影响

英文题名：Influence of Methanol Feeding Strategies on the Expression of Human Augmenter of Liver Regeneration in Recombinant Pichia pastoris

作者：平续斌[1];李志敏[1];叶勤[1];黄秀东[2];曹之舫[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]上海万兴生物制药有限公司,上海201206

年份：2008

卷号：39

期号：11

起止页码：816

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：上海市科委生物医药重点科技攻关项目(064319036)

语种：中文

中文关键词：毕赤酵母;人肝再生增强因子;甲醇流加策略;高密度发酵

外文关键词：Pichiapastoris; hALR; methanol feeding strategy; high-density fermentation

摘要：考察了5L发酵罐中三种甲醇流加策略(恒溶氧、恒比生长速率和恒甲醇浓度)对重组毕赤酵母表达人肝再生增强因子(hALR)的影响。控制恒定甲醇浓度为10g/L诱导时,甲醇比消耗速率和hALR表达速率显著高于其它两种流加策略,最终hALR表达水平为360mg/L。
The effects of three methanol feeding strategies （DO-stat, constant specific growth rate and methanol-stat） on the expression of human augmenter of liver regeneration （hALR） in recombinant Pichia pastoris were studied in a 5L bioreactor. When methanol concentration was controlled at 10g/L for induction, the specific methanol consumption rate and hALR production rate were significantly higher than those obtained with other strategies, and the final hALR expression level reached to 360mg/L.