文献类型：期刊文献

英文题名：Hyperproduction of ginseng saponin and polysaccharide by high density cultivation of Panax notoginseng cells

作者：Zhang, Yi-Heng[1]; Zhong, Jian-Jiang[2,3,4]

机构：[1]E CHINA UNIV SCI & TECHNOL,STATE KEY LAB BIOREACTOR ENGN,BIOCHEM ENGN RES INST,SHANGHAI 200237,PEOPLES R CHINA;[2]E CHINA UNIV SCI & TECHNOL,NATL ENGN RES CTR BIOTECHNOL SHANGHAI,SHANGHAI 200237,PEOPLES R CHINA

年份：1997

卷号：21

期号：1

起止页码：59

外文期刊名：ENZYME AND MICROBIAL TECHNOLOGY

收录：;EI(收录号：1997373735770);WOS:【SCI-EXPANDED(收录号:WOS:A1997XG48700011)】；

语种：英文

外文关键词：fedbatch culture; ginseng saponin; ginseng polysaccharide; high-density suspension cultivation; Panax notoginseng; plant cell culture; metabolite production

摘要：A high-density culture (HDC) of Panax notoginseng cells for simultaneous production of ginseng saponin (secondary metabolite) and polysaccharide (primary metabolite) was performed in Erlenmeyer flasks. An increase in the initial phosphate concentration lip to 3.75 mM was found to be beneficial to the production of these two useful metabolites. The most suitable medium was ascertained to be Murashige and Skoog medium with 1 mu M Cu2+, 3.75 mM phosphate, and 50 g sucrose l(-1). The inoculum size in a range of 2.53-10.2 g dry weight (DW) l(-1) had significant effect on the cell growth and metabolite accumulation, By investigating the kinetics and optimizing the environmental conditions of the cell cultures, a cell density of 24 g DW l(-1) was obtained in a batch culture. Furthermore, a fedbatch cultivation was attempted to further enhance the cell density and metabolite production. A high cell concentration of 35 g DW l(-1) and high production of ginseng saponin (1.57 g l(-1)) and polysaccharide (5.22 g l(-1)) were successfully achieved by intermittent feeding of sucrose to keep its medium level below 20 g l(-1) in the later part of the cultivation. As observed, the cell sedimentation volume reached almost 100%. The cell density attained may be the upper limit of the suspension cultures of P. notoginseng. The productivities of ginseng saponin and polysaccharide were 2.8 and 3.4-fold higher than those obtained in a conventional batch cultivation. (C) 1997 by Elsevier Science Inc.