文献类型：专利

英文题名：Detecting Sclerotinia sclerotiorum in sample comprises inoculating sample into liquid or solid culture medium having specific detection reagent, culturing and observing mycelial colonies or liquid culture color by visual or using microscope

作者：HUANG Q;LI G;QIAN X;XU W;TAO L;FENG Z

机构：[1]UNIV EAST CHINA SCI & TECHNOLOGY

申请号：CN103571910-A

申请日：2013-11-14

公开日：2014-02-12

语种：英文

收录：DERWENT

摘要：NOVELTY - Detecting Sclerotinia sclerotiorum in sample comprises (a) inoculating sample into liquid or solid culture medium containing specific detection reagent and culturing under suitable conditions, where the specific detection reagent is 3-(2-pyridyl)methyl-2-(4-chlorophenyl)imino thiazolidine or its imino-group containing thiazolidine derivative (I), and (b) visually or microscopically observing the cultured resultant mycelial colonies or liquid culture color, if the observed colonies or mycelium shows green color, then the sample indicate presence of Sclerotinia sclerotiorum. USE - The method is useful for detecting Sclerotinia sclerotiorum in a sample (claimed). DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) extracting green pigment from Sclerotinia sclerotiorum fungus mycelium comprising extracting green pigment from mycelium by using low alcohol under acidic conditions; (2) culturing and detecting Sclerotinia sclerotiorum culture medium comprising 3-(2-pyridyl)methyl-2-(4-chlorophenyl)imino thiazolidine or its imino group containing thiazolidine derivatives; and (3) 3-(2-pyridyl)methyl-2-(4-chlorophenyl)imino thiazolidine or its imino group containing thiazolidine derivatives useful in specific detection of Sclerotinia sclerotiorum and reducing Sclerotinia sclerotiorum virulence.