文献类型：期刊文献

英文题名：Specific detection of IMP-1 β-lactamase activity using a trans cephalosporin-based fluorogenic probe

作者：Hu, Liqiang[1];Liu, Runqiu[1];Ma, Zheng[1];Yu, Tao[1];Li, Ziyao[1];Zou, Yingqiu[1];Yuan, Chang[2];Chen, Fangfang[1];Xie, Hexin[1]

机构：[1]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Sch Pharm, State Key Lab Bioreactor Engn,Shanghai Key Lab Ne, Shanghai 200237, Peoples R China;[2]Shanghai Soong Ching Ling Sch, Shanghai 201703, Peoples R China

年份：2021

卷号：57

期号：99

起止页码：13586

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;EI(收录号：20215111372104);WOS:【SCI-EXPANDED(收录号:WOS:000723810700001)】；

基金：The authors acknowledge funding support from the NSFC-BRICS (81861148020), the Research Program of State Key Laboratory of Bioreactor Engineering, and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Amino acids - Probes

摘要：A fluorogenic probe for the specific detection of IMP-1 beta-lactamase activity has been developed. This imaging reagent features a unique trans-acetylamino cephalosporin as an enzymatic recognition moiety, exhibiting excellent selectivity to IMP-1 beta-lactamase over other beta-lactamases, including serine- and metallo-beta-lactamases. The selective activation of the probe by IMP-1 beta-lactamase leads to over 30-fold enhancement in the fluorescence intensity, which allows enzyme activity to be reported with high sensitivity.