文献类型：期刊文献

英文题名：Rational design of medium supplementation strategy for improved influenza viruses production based on analyzing nutritional requirements of MDCK Cells

作者：Huang, Ding[1];Kang Xia-Hou[1];Liu, Xu-Ping[1];Zhao, Liang[1];Fan, Li[1];Ye, Zhaoyang[1];Tan, Wen-Song[1];Luo, Jian[2];Chen, Ze[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Inst Biol Prod Co Ltd, Shanghai 200052, Peoples R China

年份：2014

卷号：32

期号：52

起止页码：7091

外文期刊名：VACCINE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000348020200012)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 21106045 and 21206040), the National High Technology Research and Development Program of China (863 Program) (No. 2012AA02A303), the National Science and Technology Major Project (No. 2013ZX10004003-003-003), the Fundamental Research Funds for the Central Universities (WF1214035).

语种：英文

外文关键词：Influenza virus; MDCK cells; Metabolism; Nutritional requirements

摘要：Influenza vaccine production using cell culture technology has become popular nowadays. However, to meet the ever increasing demand of influenza vaccine, it is prerequisite to improve the yield of influenza virus in cells. To achieve this, in the present study, the nutritional requirements of MOCK cells in the virus production process were analyzed and a nutrient-feeding strategy was developed accordingly. Based on the consumption rates and corresponding concentration optimization, glucose and fast metabolized amino acids were supplemented into the maintaining medium at the time of infection. Compared with the non-supplemented culture, the average cell specific death rate during 0-48 h post-infection was 0.013 h(-1), which was 40.91% lower in the nutrient-supplemented culture. Total virus titer, HA antigen protein concentration and cell-specific virus yield were (1.88 +/- 0.23) x 10(3) HAunits/50 mu L, 11.70 +/- 0.22 mu g/mL and (10.06 +/- 1.16) x 10(3) virions/cell, respectively, which were 84.04 +/- 22.50%, 31.46 +/- 2.87% and 86.64 +/- 25.81% higher than those in the control, respectively. These data showed that the appropriate supplementation of nutrients,during virus production process could reduce cell death, and improve cell-specific virus yield and total influenza virus output. This study laid foundation for the development of cell culture technology for influenza vaccine production. (C) 2014 Elsevier Ltd. All rights reserved.