文献类型：期刊文献

英文题名：Molecular cloning and functional characterization of an ATP-binding cassette transporter OtrC from Streptomyces rimosus

作者：Yu, Lan[1,2]; Yan, Xiangyun[1]; Wang, Long[1]; Chu, Ju[1]; Zhuang, Yingping[1]; Zhang, Siliang[1]; Guo, Meijin[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China; [2] School of Life and Science, Huzhou Teachers College, Zhejiang, Huzhou, 313000, China

年份：2012

卷号：12

外文期刊名：BMC Biotechnology

收录：EI(收录号：20130215880789)

语种：英文

外文关键词：Clone cells - Cloning - Escherichia coli

摘要：Background: The otrC gene of Streptomyces rimosus was previously annotated as an oxytetracycline (OTC) resistance protein. However, the amino acid sequence analysis of OtrC shows that it is a putative ATP-binding cassette (ABC) transporter with multidrug resistance function. To our knowledge, none of the ABC transporters in S. rimosus have yet been characterized. In this study, we aimed to characterize the multidrug exporter function of OtrC and evaluate its relevancy to OTC production.Results: In order to investigate OtrC's function, otrC is cloned and expressed in E. coli The exporter function of OtrC was identified by ATPase activity determination and ethidium bromide efflux assays. Also, the susceptibilities of OtrC-overexpressing cells to several structurally unrelated drugs were compared with those of OtrC-non-expressing cells by minimal inhibitory concentration (MIC) assays, indicating that OtrC functions as a drug exporter with a broad range of drug specificities. The OTC production was enhanced by 1.6-fold in M4018 (P = 0.000877) and 1.4-fold in SR16 (P = 0.00973) duplication mutants, while it decreased to 80% in disruption mutants (P = 0.0182 and 0.0124 in M4018 and SR16, respectively).Conclusions: The results suggest that OtrC is an ABC transporter with multidrug resistance function, and plays an important role in self-protection by drug efflux mechanisms. This is the first report of such a protein in S. rimosus, and otrC could be a valuable target for genetic manipulation to improve the production of industrial antibiotics. ? 2012 Yu et al.; licensee BioMed Central Ltd.