文献类型：期刊文献

英文题名：Catalytic conversion of corncob to furfuryl alcohol in tandem reaction with tin-loaded sulfonated zeolite and NADPH-dependent reductase biocatalyst

作者：Li, Yuan-Yuan[1];Li, Qing[2];Zhang, Peng-Qi[2];Ma, Cui-Luan[1,2,3];Xu, Jian-He[3];He, Yu-Cai[1,2,3,4]

机构：[1]Changzhou Univ, Lab Bioresourse & Bioproc, Adv Catalysis & Green Mfg Collaborat Innovat Ctr, Jiangsu Key Lab Adv Catalyt Mat & Technol,Natl Lo, Changzhou, Jiangsu, Peoples R China;[2]Hubei Univ, Lab Biomass & Bioenergy,Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn,Hubei Ke, Hubei Collaborat Innovat Ctr Green Transformat Bi, Wuhan, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[4]Huaiyin Normal Univ, Jiangsu Key Lab Biomass Based Energy & Enzyme Tec, Huaian, Peoples R China

年份：2021

卷号：320

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20204409417134);WOS:【SCI-EXPANDED(收录号:WOS:000600044100008)】；

基金：The authors are grateful for the financial support from the National Key Research and Development Program of China (No. 2019YFA09005000), the National Natural Science Foundation of China (No. 21978072), the Open Project of State Key Laboratory of Biocatalysis and Enzyme Engineering (China), and the Open Project of Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology (No. BEETKB1902).

语种：英文

外文关键词：Corncob; Sn-zeolite catalyst; Furfuryl alcohol; Reductase; Recombinant E. coli CG-19

摘要：In this study, tin-loaded sulfonated zeolite (Sn-zeolite) catalyst was synthesized for catalysis of raw corncob (75.0 g/L) to 103.0 mM furfural at 52.3% yield in water (pH 1.0) at 170 degrees C. This corncob-derived furfural was subsequently biotransformed with recombinant E. coli CG-19 cells coexpressing NADPH-dependent reductase and glucose dehydrogenase at 35 degrees C by supplementary of glucose (1.5 mol glucose/mol furfural), sodium dodecyl sulfate (0.50 mM) and NADP(+) (1.0 mu mol NADP(+)/mmol furfural) in the aqueous catalytic media (pH 7.5). Both sodium dodecyl sulfate (0.50 mM) and Sn4+ (1.0 mM) could promote reductase activity by 1.4-folds. Within 3 h, furfural was wholly catalyzed into furfuryl alcohol. By combining chemical catalysis with Sn-zeolite and biocatalysis with CG-19 cells in one-pot, an effective and sustainable process was established for tandemly catalyzing renewable biomass into furfuryl alcohol under environmentally-friendly way.