文献类型：会议论文

英文题名：A new reversed-phase/strong anion-exchange mixed-mode stationary phase based on polar-copolymerized approach and its application in the enrichment of aristolochic acids

作者：Wei, Jie[1];Guo, Zhimou[2];Zhang, Pinjie[1];Zhang, Feifang[1];Yang, Bingcheng[1];Liang, Xinmiao[1,2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

会议论文集：37th International Symposium on High-Performance Liquid-Phase Separations and Related Techniques/18th NSEC/2nd DISEC

会议日期：OCT 08-11, 2011

会议地点：Dalian, PEOPLES R CHINA

语种：英文

外文关键词：Solid-phase extraction; Mixed-mode chromatography; Strong anion exchange; Polar-copolymerized; Aristolochic acids; Purification

摘要：A novel silica-based reversed-phase/strong anion-exchange mixed-mode stationary phase named C18SAX was synthesized based on the polar-copolymerized approach. C18SAX stationary phase showed excellent compatibility with 100% aqueous mobile phase and comparable performance with commercial SunFire (TM) C18 column in terms of column efficiency and methylene selectivity. And the results indicated that hydrophobic interaction and anion-exchange mechanism dominate the separation process of aristolochic acids. The utility of C18SAX material-based SPE was demonstrated for the enrichment of aristolochic acids extracted from Caulis Aristochiae Manshuriensis. The SPE method was developed rapidly and simply by HPLC evaluation. Under the optimized SPE conditions, the average recoveries of aristolochic acid land aristolochic acid II in crude sample were 72.36% and 105.59%, with RSDs of 5.77% and 1.13%, respectively. The complexity of sample matrices was significantly simplified after the SPE procedure and further preparation and purification of target compounds was performed on a preparative C18TDE column. Four aristolochic acids, including aristolochic acid I (31.8 mg), II (8.9 mg), IIIa (6.1 mg) and IVa (4.3 mg) were purified with HPLC purities all above 93%. The results proved that the aristolochic acids could be efficiently enriched by C18SAX material and easily eluted in acidic conditions, indicating its great potential in the enrichment of acidic compounds from complex matrices. (C) 2012 Elsevier B.V. All rights reserved.