文献类型：期刊文献

英文题名：Enhancement of VEGF-Mediated Angiogenesis by 2-N,6-O-Sulfated Chitosan-Coated Hierarchical PLGA Scaffolds

作者：Yu, Yuanman[1,3];Chen, Jie[1,3];Chen, Rui[1,3];Cao, Lingyan[1,3];Tang, Wei[1,3];Lin, Dan[1,3];Wang, Jing[1,3];Liu, Changsheng[1,2,3]

机构：[1]E China Univ Sci & Technol, Minist Educ, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Minist Educ, Engn Res Ctr Biomed Mat, Shanghai 200237, Peoples R China

年份：2015

卷号：7

期号：18

起止页码：9982

外文期刊名：ACS APPLIED MATERIALS & INTERFACES

收录：;EI(收录号：20152000857721);WOS:【SCI-EXPANDED(收录号:WOS:000354906500077)】；

基金：We express our gratitude for the financial support from the National Basic Research Program of China (973 Program, No. 2012CB933600), the National Natural Science Foundation of China (Grant Nos. 31271011, 31330028, and 31470923), and the National Science and Technology Support Program (Grant No, 2012BAI17B02). This study is also supported by New Century Excellent Talents in University (Grant No. NCET-12-0856). Shanghai Synchrotron Radiation Facility is also gratefully acknowledged.

语种：英文

外文关键词：Scaffold; poly(lactic-co-glycolic acid); sulfated chitosan; vascular endothelial growth factor; angiogenesis

摘要：Rapid and controlled vascularization within scaffolds remains one of the key limitations in tissue engineering applications. This study describes the fabrication and characterization of 2-N,6-O-sulfated chitosan (26SCS)-coated hierarchical scaffold composed of poly(lactic-co-glycolic acid) (PLGA) microspheres, as a desirable vehicle for vascular endothelial growth factor (VEGF) delivery and consequent angiogenic boosting in vitro. Owing to the hierarchical porous structure and high affinity between VEGF and 26SCS, the 26SCS-modified PLGA (S-PLGA) scaffold possesses excellent entrapment and sustained release of VEGF. Using human umbilical vein endothelial cells (HUVECs) as a cell model, the VEGF-loaded S-PLGA scaffold shows desirable cell viability and attachment. The bioactivity of released VEGF is validated by intracellular nitric oxide secretion and capillary tube formation, demonstrating the improved capacity of VEGF-mediated pro-angiogenesis ascribed to 26SCS incorporation. Such a strategy will afford an effective method to prepare a scaffold with promoted angiogenesis.