文献类型：期刊文献

英文题名：Harnessing a previously unidentified capability of bacterial allosteric transcription factors for sensing diverse small molecules in vitro

作者：Cao, Jiaqian[1,2];Yao, Yongpeng[1,2];Fan, Keqiang[1,2];Tan, Gaoyi[3];Xiang, Wensheng[4];Xia, Xuekui[5];Li, Shanshan[4];Wang, Weishan[1,2];Zhang, Lixin[1,2,3,6]

机构：[1]State Key Lab Microbial Resources, Beijing 100101, Peoples R China;[2]Chinese Acad Sci, Key Lab Pathogen Microbiol & Immunol, Inst Microbiol, Beijing 100101, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China;[5]Qilu Univ Technol, Biol Inst, Key Lab Biosensor Shandong Prov, Shandong Acad Sci, Jinan 250013, Shandong, Peoples R China;[6]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266061, Peoples R China

年份：2018

卷号：4

期号：11

外文期刊名：SCIENCE ADVANCES

收录：;EI(收录号：20184906202107);WOS:【SCI-EXPANDED(收录号:WOS:000452212000050)】；

基金：This work was supported by the National Natural Science Foundation of China (grant nos. 31430002, 31770055, 31570031, 31772242, 31600017, and 31500690), the Ministry of Science and Technology of China (grant nos. 2017YFD020120 and 2015ZX09J15102-004), the Youth Innovation Promotion Association of CAS (grant no. 2016087), the International Partnership Program of Chinese Academy of Sciences (grant no. 153211KYSB20170014), the Fundamental Research Funds for the Central Universities (grant no. 22221818014), the Major Basic Program of the Natural Science Foundation of Shandong Province (grant no. ZR2017ZB0206), and the Shandong Taishan Scholar Program of China (to L.Z.). Funding for open-access charge was provided by the National Natural Science Foundation of China.

语种：英文

外文关键词：Biosensors - Transcription - DNA - Binding sites - Signal transduction

摘要：A plethora of bacterial allosteric transcription factors (aTFs) have been identified to sense a variety of small molecules. Introduction of a novel aTF-based approach to sense diverse small molecules in vitro will signify a broad series of detection applications. Here, we found that aTFs could interact with their nicked DNA binding sites. Building from this new finding, we designed and implemented a novel aTF-based nicked DNA template-assisted signal transduction system (aTF-NAST) by using the competition between aTFs and 14 DNA ligase to bind to the nicked DNA. This aTF-NAST could reliably and modularly transduce the signal of small molecules recognized by aTFs to the ligated DNA signal, thus enabling the small molecules to be measured via various mature and robust DNA detection methods. Coupling this aTF-NAST with three DNA detection methods, we demonstrated nine novel biosensors for the detection of an antiseptic 4-hydroxybenzoic acid, a disease marker uric acid and an antibiotic tetracycline. These biosensors show impressive sensitivity and robustness in real-life analysis, highlighting the great potential of our aTF-NAST for biosensing applications.