文献类型：期刊文献

英文题名：Metabolic engineering of Escherichia coli for efficient production of O-succinyl-L-homoserine

作者：Liu, Longmei[1];Gu, Jieyun[1];Lyu, Xiaomei[2];Gu, JiaLi[3];Liu, Qinghai[1];Ma, Yu-Shu[1];Tao, Xin-Yi[1];Liu, Min[1];Wei, Dong-Zhi[1]

机构：[1]East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China;[3]Huzhou Univ, Coll Life Sci, Huzhou 313000, Peoples R China

年份：2026

卷号：232

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20261820628136);WOS:【SCI-EXPANDED(收录号:WOS:001760105800001)】；

基金：This study was supported by Shanghai Science and Technology Development Funds (No. 24HC2810400) , the Natural Science Founda-tion of Shanghai (No. 21ZR1417200) , and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering. No other competing interests are declared.

语种：英文

外文关键词：O-Succinyl-L-homoserine; Escherichia coli; Self-regulating promoter; Metabolic engineering; Flagellar system

摘要：The flagellar system of Escherichia coli has a three-tier transcriptional network for temporal control of flagellar synthesis. This work used the operon elements from flagellar system to build an auto-inducible system for balancing cell growth and O-Succinyl-L-homoserine (OSH) production in E. coli. The initial OSH producing strain was created by regulating the L-threonine pathway using the self-regulatory promoter PfliC from E. coli MG1655, and blocking the downstream degradation pathway. Subsequently, the negative feedback inhibition of key enzyme was relieved, followed by modifying the glucose uptake system, enhancing precursor, coenzyme, and ATP supply. Finally, the self-regulatory promoters from flagellar system were systematically screened, with PfliF identified as the optimal promoter, which increased OSH titer from 85.1 g/L to 138.1 g/L in 5 L fermenter, with yield and productivity values of 0.54 g/g and 1.44 g/L/h, respectively, representing the highest titer reported to date. The work provides valuable insights for industrial OSH production.