文献类型：期刊文献

中文题名：D-乳酸合成阻断对肺炎克雷伯氏菌的1,3-丙二醇发酵的影响

英文题名：Influence of blocking formation of D-lactic acid on 1,3-propanediol fermentation by Klebsiella pneumoniae

作者：周佳佳[1];高黎荣[1];刘龙飞[1];张书翠[1];付水林[1];宫衡[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2014

卷号：44

期号：4

起止页码：24

中文期刊名：工业微生物

外文期刊名：Industrial Microbiology

收录：CSCD:【CSCD_E2013_2014】；

语种：中文

中文关键词：肺炎克雷伯氏菌;D-乳酸脱氢酶;1,3-丙二醇;Red同源重组

外文关键词：Klebsiella pneumoniae ; D-lactate dehydrogenase ; 1,3-propanediol ; Red recombination

摘要：利用Red同源重组技术,快速敲除肺炎克雷伯氏菌中的编码D-乳酸脱氢酶的两个基因——ldhA和dld,获得KG1-1和KG1-2两个突变株,并研究了敲除编码D-乳酸脱氢酶基因丧失合成D-乳酸的KG1-1菌株的1,3-PD产量和菌体生长变化,实验结果表明乳酸合成缺失对现有工艺1,3-丙二醇发酵无影响。
The impact of blocking D-lactate formation by direct knocking out the genes of D-lactic dehydrogenase in Klebsiella pneumoniae was investigated. The two genes of ldhA and dld were knocked out separately to construct two mutants of KG1-1 and KG1-2. The results showed that KGI-1 was a D-lactate deficient mutant, while its 1,3-PD production and cell growth were not influenced on the present technology.