文献类型：期刊文献

英文题名：Preparative separation of the polar part from the rhizomes of Anemarrhena asphodeloides using a hydrophilic C18 stationary phase

作者：Cai, Jianfeng[1];Xin, Huaxia[1];Cheng, Lingping[1];Fu, YanHui[2];Jiang, Dasen[1];Feng, Jiatao[3];Fu, Qing[1];Jin, Yu[1];Liang, Xinmiao[1,3]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China;[2]Hai Nan Normal Univ, 99 South Longkun Rd, Haikou 571127, Hainan, Peoples R China;[3]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116203, Peoples R China

年份：2017

卷号：1063

起止页码：149

外文期刊名：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

收录：;EI(收录号：20173604108191);WOS:【SCI-EXPANDED(收录号:WOS:000412255000022)】；

基金：This work was supported by National Natural Science Funds (81403100) and Fundamental Research Funds for the Central Universities (222201717016).

语种：英文

外文关键词：Hydrophilic C18 stationary phase; Anemarrhena asphodeloides; Polar part; Preparative high performance liquid; chromatography

摘要：The goal of this study was to develop a method that utilized a hydrophilic C18 stationary phase in the preparative high performance liquid chromatography to isolate the polar part from the rhizomes of Anemarrhena asphodeloides. The results showed that an initial mobile phase of pure water for the separation could greatly increase the retention and solubility of the polar compounds at the preparative scale. Introducing polar groups on the surface of the hydrophilic C18 column together with the use of optimized mobile phase compositions improved the column separation selectivity for polar compounds. Eleven previously undescribed compounds in Anemarrhena asphodeloides were obtained, indicating that the method developed in this study would facilitate the purification and separation of the polar part of traditional Chinese medicines.