文献类型：期刊文献

英文题名：Metabolic Engineering of Filamentous Fungus Trichoderma reesei for Itaconic Acid Production

作者：Chen, Yumeng[1];Wang, Ziwei[1];Chen, Cangcang[1];Xiao, Sheng[1];Lv, Jia[1];Lin, Ling[1];Liu, Jiayu[1];Li, Xinrui[1];Wang, Wei[1];Wei, Dongzhi[1]

机构：[1]East China Univ Sci & Technol, Luhua Suo Inst, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2025

卷号：73

期号：8

起止页码：4716

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20250817908553);WOS:【SCI-EXPANDED(收录号:WOS:001424814900001)】；

基金：This work was supported by the National Key Research and Development Program of China [Grant 2022YFA0912300], the National Natural Science Foundation of China [Grant 32370093], the Natural Science Foundation of Shanghai [Grants 22ZR1417600 and 23ZR1414800], the Chenguang Program of the Shanghai Education Development Foundation, Shanghai Municipal Education Commission [Grant 21CGA34], and the Open Funding Project of the State Key Laboratory of Bioreactor Engineering [Grant 2022].

语种：英文

外文关键词：Trichoderma reesei; itaconic acid (IA); metabolic engineering; cell factory

摘要：Itaconic acid (IA) is a multifunctional platform chemical with numerous biological functions. Here, Trichoderma reesei was engineered as a remarkable cell factory to produce IA. Heterologous overexpression of the mitochondrial tricarboxylate transport protein and cis-aconitate decarboxylase from Aspergillus terreus in T. reesei initiated IA production with a titer of 20 g/L. By increasing the copy number of mttA and cadA and the overexpression of the plasma membrane transporter proteins (MFSA), the titer of IA reached 56.7 g/L. The precursor synthesis pathway of IA was overexpressed by the overexpression of aconitase and citrate synthase, and the IA competition pathway was blocked by the deletion of the P450 monooxygenase gene cyp3 to further enhance IA production. The final strain resulted in a final IA titer of 93.5 g/L through fed-batch fermentation in a 1 L bioreactor. Our study demonstrates that T. reesei can serve as a relevant platform in industry for IA production.