文献类型：期刊文献

中文题名：1,8-萘酰亚胺类荧光探针对肼的检测

英文题名：1,8-Naphthalimide-based Fluorescent Probe for the Detection of Hydrazine

作者：常炜婕[1];何雨婷[1];许方龙[1];张维冰[1];钱俊红[1]

机构：[1]上海市功能性材料重点实验室,华东理工大学化学与分子工程学院,上海200237

年份：2025

卷号：44

期号：12

起止页码：2601

中文期刊名：分析测试学报

外文期刊名：Journal of Instrumental Analysis

收录：;北大核心:【北大核心2023】；

基金：国家重点研发计划(2022YFD1700400)。

语种：中文

中文关键词：荧光探针;1,8-萘酰亚胺;肼;溶酶体定位;细胞成像

外文关键词：fluorescent probes;1,8-naphthalimide;hydrazine;lysosome-targeting;cellular imaging

摘要：基于Gabriel反应,在萘酰亚胺的4-位引入邻苯二甲酰亚胺、亚胺位引入溶酶体定位基团吗啉,合成了探针NMA和NMB用于对肼的检测。实验结果表明两种探针对肼均有较强的光谱响应,其中NMA对肼的响应更加灵敏、快速。肼的加入使NMA的吸收光谱红移100 nm,其在527 nm处的荧光信号最大增强约500倍。在5.0~70μmol/L范围内,NMA的荧光强度与肼的浓度呈现良好的线性关系,对肼的检出限为1.83μmol/L。此外,通过高效液相色谱和密度泛函理论计算验证了探针NMA对肼的响应机理。细胞成像结果表明NMA能够实现对溶酶体内肼的荧光成像。
Based on the Gabriel reaction,two fluorescent probes,NMA and NMB,were synthesized for hydrazine detection.The probes were constructed with naphthalimide as the fluorophore,a phthalimide at the 4-position selected as the receptor and a morpholine at imide site as a lysosome-targeted group.Experimental results demonstrated that both probes exhibited evidently spectral responses to hydrazine.Compared to NMB,NMA displayed better performance such as higher sensitivity and faster response.The addition of hydrazine resulted in~100 nm red shift in the absorption of NMA,companied with 500-fold fluorescence enhancement.The fluorescence intensity of NMA showed a good linear relationship with hydrazine concentration in the range of 5.0-70μmol/L,with a detection limit(3σ/k)of 1.83μmol/L.The response mechanism of NMA to hydrazine was validated by HPLC and theoretical calculations.Confocal imaging experiments demonstrated that NMA could realize imaging lysosomal hydrazine.