文献类型：期刊文献

英文题名：A water-soluble boronate-based fluorescent probe for the selective detection of peroxynitrite and imaging in living cells

作者：Sun, Xiaolong[1];Xu, Qingling[2];Kim, Gyoungmi[2];Flower, Stephen E.[1];Lowe, John P.[1];Yoon, Juyoung[2];Fossey, John S.[3];Qian, Xuhong[4];Bull, Steven D.[1];James, Tony D.[1]

机构：[1]Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England;[2]Ewha Womans Univ, Dept Chem & Nano Sci, Global Top Program 5, Seoul 120750, South Korea;[3]Univ Birmingham, Sch Chem, Birmingham B15 2TT, W Midlands, England;[4]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2014

卷号：5

期号：9

起止页码：3368

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20143218033295);WOS:【SCI-EXPANDED(收录号:WOS:000340695800002)】；

基金：TDJ and XS are grateful for financial support from China Scholarship Council (CSC) and University of Bath Full Fees Scholarship. The Catalysis And Sensing for our Environment (CASE) network is thanked for research exchange opportunities. JSF, SDB and TDJ thank ECUST for guest professorships. JSF thanks the University of Birmingham for support. JY thanks the support from the National Research Foundation of Korea (NRF) grants funded by the Korean government (MSIP) (no. 2012R1A3A2048814).

语种：英文

外文关键词：Fructose - Lanthanum compounds - Cytology - Fluorescence - Fluorescence spectroscopy

摘要：A water-soluble boronate-based fluorescent probe was evaluated for the detection of peroxynitrite (ONOO-) in the presence of a monosaccharide. The enhanced fluorescence of the probe when bound with o-fructose was switched off in the presence of peroxynitrite. In contrast, other reactive oxygen/nitrogen species (ROS/RNS) led to only slight fluorescence decreases due to protection by an internal N-B interaction. The interaction of the probe with o-fructose not only strengthens the fluorescence signal, but also protects the boronic acid from oxidation by other ROS/RNS. Therefore, under conditions generating various ROS/RNS, the boronate-based saccharide complex preferentially reacts with peroxynitrite. The probe was used in cell imaging experiments for the detection of endogenous and exogenous peroxynitrite. The sensor displays good "on-off" responses towards peroxynitrite, both in RAW 264.7 cells and HeLa cells.