文献类型：期刊文献

英文题名：Microbe-focused glycan array screening platform

作者：Geissner, Andreas[1,2,9];Reinhardt, Anika[1,2,10];Rademacher, Christoph[1,2];Johannssen, Timo[1,2];Monteiro, Joao[3,4];Lepenies, Bernd[1,2,3,4];Thepaut, Michel[5,6,7];Fieschi, Franck[5,6,7];Mrazkova, Jana[8];Wimmerova, Michaela[8];Schuhmacher, Frank[1,2];Goetze, Sebastian[1,2,11];Gruenstein, Dan[1,2];Guo, Xiaoqiang[1,2,12];Hahm, Heung Sik[1,2,13];Kandasamy, Jeyakumar[1,14];Leonori, Daniele[1,15];Martin, Christopher E.[1,2,16];Parameswarappa, Sharavathi G.[1,17];Pasari, Sandip[1,18];Schlegel, Mark K.[1,19];Tanaka, Hidenori[1,20];Xiao, Guozhi[1,21];Yang, You[1,22];Pereira, Claney L.[1,17];Anish, Chakkumkal[1,23];Seeberger, Peter H.[1,2]

机构：[1]Max Planck Inst Colloids & Interfaces, Dept Biomol Syst, D-14424 Potsdam, Germany;[2]Free Univ Berlin, Inst Chem & Biochem, D-14195 Berlin, Germany;[3]Univ Vet Med Hannover, Immunol Unit, D-30559 Hannover, Germany;[4]Univ Vet Med Hannover, Res Ctr Emerging Infect & Zoonoses, D-30559 Hannover, Germany;[5]Univ Grenoble Alpes, Inst Biol Struct, F-38044 Grenoble, France;[6]CNRS, Inst Biol Struct, F-38044 Grenoble, France;[7]Commissariat Energie Atom & Energies Alternat, Inst Biol Struct, F-38044 Grenoble, France;[8]Masaryk Univ, Cent European Inst Technol, Brno 62500, Czech Republic;[9]Univ British Columbia, Dept Chem, Vancouver, BC V6T 1Z1, Canada;[10]Kyoto Univ, Dept Life Sci Frontiers, Ctr iPS Cell Res & Applicat, Kyoto 6068507, Japan;[11]Univ Koblenz Landau, Fac Nat & Environm Sci 7, D-76829 Landau, Germany;[12]Clariant Innovat Ctr, D-65929 Frankfurt, Germany;[13]Univ Virginia, Dept Chem, Charlottesville, VA 22904 USA;[14]Indian Inst Technol, Dept Chem, Varanasi 221005, Uttar Pradesh, India;[15]Univ Manchester, Sch Chem, Manchester M13 9PL, Lancs, England;[16]Bachem AG, CH-4416 Bubendorf, Switzerland;[17]Vaxxilon Deutschland GmbH, D-12489 Berlin, Germany;[18]Indian Inst Sci Educ & Res, Pune 411008, Maharashtra, India;[19]Alnylam Pharmaceut, Cambridge, MA 02142 USA;[20]Kochi Univ, Ctr Sci Res, Oceanog Sect, Otsu 200, Nankoku, Kochi 7838503, Japan;[21]Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China;[22]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[23]Janssen Pharmaceut Johnson & Johnson, Bacterial Vaccines Discovery & Early Dev, NL-2333 CK Leiden, Netherlands

年份：2019

卷号：116

期号：6

起止页码：1958

外文期刊名：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000457731900027)】；

基金：We thank Prof. Ten Feizi, director of the Glycosciences Laboratory at Imperial College London, for cooperation and support and coworkers, especially Microarray Project Leader Dr. Yan Liu, in enabling library comparison; Dr. Lenka Malinovska for providing fluorescently labeled Burkholderia lectins for initial exploratory studies; all chemists involved in preparing the many defined glycans displayed on the array; and Dr. Allison Berger and Dr. Benjamin Schumann for critically editing the manuscript. Healthy human serum samples were a kind gift of Dr. Andreas Bergmann (Sphingotec GmbH). This work was supported by the Max Planck Society, Zukunftsfonds Berlin, European Regional Development Fund Grant GlyCarDiag, European Research Council Advanced Grant AUTOHEPARIN, German Federal Ministry for Education and Research Grant 0315447, and the Korber Foundation. A.R. was supported by a graduate fellowship of the Studienstiftung des Deutschen Volkes. C.R. is supported by the German Research Foundation through Emmy Noether Fellowship RA1944/2-1, B.L. by Deutsche Forschungsgemeinschaft Grant LE 2498/6-1, and M.T., F.F., B.L., and J.M. by the European Union's Horizon 2020 Research and Innovation Program (Marie Sklodowska-Curie Grant 642870, ETN-Immunoshape). Expression and purification of Burkholderia lectins was supported by the project CEITEC 2020 (LQ1601) from the Ministry of Education, Youth and Sports of the Czech Republic. For DC-SIGN-T production, this work used the Multistep Protein Purification Platform (MP3) of the Grenoble Instruct Centre (ISBG; UMS 3518 CNRS-CEA-UJF-EMBL) with support from FRISBI Grant ANR-10-INSB-05-02 and GRAL Grant ANR-10-LABX-49-01 within the Grenoble Partnership for Structural Biology. F.F. also acknowledges the support of the French Agence Nationale de la Recheche (ANR) for Glyco@Alps(ANR- 15-IDEX-02).

语种：英文

外文关键词：glycan arrays; microbial antigens; immune receptors; antiglycan antibodies; bacterial lectins

摘要：Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein-glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.