文献类型：期刊文献

英文题名：Convenient and Efficient FRET Platform Featuring a Rigid Biphenyl Spacer between Rhodamine and BODIPY: Transformation of 'Turn-On' Sensors into Ratiometric Ones with Dual Emission

作者：Yu, Haibo[1];Xiao, Yi[1];Guo, Haiying[1];Qian, Xuhong[2]

机构：[1]Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China;[2]E China Univ Sci & Technol, Biol Chem Lab, Shanghai 200237, Peoples R China

年份：2011

卷号：17

期号：11

起止页码：3179

外文期刊名：CHEMISTRY-A EUROPEAN JOURNAL

收录：;EI(收录号：20111013717315);WOS:【SCI-EXPANDED(收录号:WOS:000288098800019)】；

基金：We thank the NSF of China (no. 20876022), the Fundamental Research Funds for the Central University (DUT10ZD114) and the Program for Changjiang Scholars and Innovative Research Team in University (IRT0711) for financial support. We also thank Dr. Liji Jin (Dalian University of Technology) for help with cell culture experiments and Dr. Xiaolin Yuan (Dalian University) for help with confocal microscopy experiments.

语种：英文

外文关键词：donor-acceptor systems; fluorescence; FRET; mercury; sensors

摘要：We have connected a boron-dipyrromethene (BODIPY) donor to the 5' position of a tetramethylrhodamine (TMR) acceptor to form a high efficiency (over 99%) intramolecular fluorescence resonance energy transfer (FRET) cassette, BODIPY-rhodamine platform (BRP). While the good spectral overlap between the emission of BODIPY and the absorption of TMR was one favorable factor, another feature of this FRET system was the rigid and short biphenyl spacer that favored efficient through-bond energy transfer. More importantly, in this system, the 2'-carboxyl group of the rhodamine unit was preserved for the further modifications, which was as convenient as those carbonyl groups on the original rhodamines without connection to donors. For this reason, BRP is clearly differentiated from the previous ratiometric sensors based on donor rhodamine systems. To illustrate its value as a versatile platform, we introduced typical Hg2+ receptors into BRP, through convenient one-pot reactions on the 2'-carboxyl group, and successfully developed two ratiometric sensors, BRP-1 and BRP-2, with different spirocyclic receptors that recognized Hg2+ on different reaction mechanisms. Upon excitation at a single wavelength (488 nm), at which only BODIPY absorbed, both of the FRET sensors exhibited clear Hg2+-induced changes in the intensity ratio of the two strong emission bands of BODIPY and rhodamine. It should be noted that these ratiometric Hg2+ sensors exhibited excellent sensitivity and selectivity Hg2+, as well as pH insensitivity, which was similar to the corresponding (turn-on) rhodamine sensors. While both ratiometric probes were applicable for Hg2+ imaging in living cells, BRP-1 exhibited higher sensitivity and faster responses than BRP-2. Our investigation indicated that on a versatile platform, such as BRP, a large number of highly efficient ratiometric sensors for transition-metal ions could be conveniently developed.