文献类型：期刊文献

英文题名：A Novel Wick-Like Paper-Based Microfluidic Device for 3D Cell Culture and Anti-Cancer Drugs Screening

作者：Fu, Shu-xia[1];Zuo, Peng[1];Ye, Bang-Ce[1]

机构：[1]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2021

卷号：16

期号：2

外文期刊名：BIOTECHNOLOGY JOURNAL

收录：;EI(收录号：20204209369450);WOS:【SCI-EXPANDED(收录号:WOS:000579183100001)】；

基金：This study was supported by the China NSF 31571929 and 31730004, the Fundamental Research Funds for the Central Universities, and Open Funding Project of State Key Laboratory of Dairy Biotechnology (SKLDB).

语种：英文

外文关键词：3D cell culture; colorimetric analysis; drug screening; paper-based microfluidic

摘要：Paper is increasingly recognized as a portable substrate for cell culture, due to its low-cost, flexible, and special porous property, which provides a native cellular 3D microenvironment. Therefore, paper-based microfluidics has been developed for cell culture and biomedical analysis. However, the inability of continuous medium supply limits the wide application of paper devices for cell culture. Herein, a paper-based microfluidic device is developed with novel folded paper strips as wick-like structure, which is used for medium self-driven perfusion. The paper with patterns of hydrophilic channel, culture areas, and hydrophobic barrier could be easily fabricated through wax-printing. After printing, the hydrophilic paper strip at the periphery of the lower layer is then folded at 90 degrees and extended into the medium container for continuous automatic supply of medium to the cell culture area. Tumor cells cultured in the paper device are tested for anti-cancer drug screening. Visualized cell viability and chemical sensitivity testing can be achieved by colorimetry combined with simple smartphone imaging, effectively reducing precision instrument dependence. The wick paper-based microfluidic device for cell culture endows the method the advantages of lower cost, ease-of-operation, miniaturization, and shows a great potential for large-scale cell culture, antibody drug production, and efficient screening.