文献类型：期刊文献

英文题名：HPLC-electrospray tandem mass spectrometry for simultaneous quantitation of eight plasma aminothiols: Application to studies of diabetic nephropathy

作者：Jiang, Zhiting[1,2];Liang, Qiongling[1];Luo, Guoan[1,2];Hu, Ping[3];Li, Ping[4];Wang, Yiming[1]

机构：[1]Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Coll Chem & Mol Engn, Shanghai 200237, Peoples R China;[4]Chinese Japanese Friendship Hosp, Clin Med Res Inst, Beijing, Peoples R China

年份：2009

卷号：77

期号：4

起止页码：1279

外文期刊名：TALANTA

收录：;EI(收录号：20085011780592);WOS:【SCI-EXPANDED(收录号:WOS:000262761500003)】；

基金：The investigation received financial assistance from the National Basic Research Development Program of China (973 Program, Nos. 2005CB523503, 2007CB714505) and National Natural Science Foundation of China (No. 90709045).

语种：英文

外文关键词：Aminothiol; HPLC-ESI-MS/MS; Diabetic nephropathy

摘要：It was reported that Hcy was related to the development of kidney disease, but it remains unknown whether Hcy is an independent biomarker for diabetic nephropathy. Analytical method for simultaneous determination of aminothiols among the Hcy metabolic cycle is desirable to discover other potential biomarkers. A high-performance liquid chromatography-electrospray tandem mass spectrometric (HPLC-ESI-MS/MS) method was established for simultaneous quantitation of Cysteine (Cys), total homocysteine (tHcy). S-adenosylrnethionine (SAM), S-adenosylhomocysteine (SAH), cystathionine (Cysta), methionine (Met), glutathione (GSH) and cysteinylglycine (Cys-gly) in plasma with N-(2-mercaptopropionyl)-glycine (MPG) as internal standard. The method had simple pretreatment without derivatization and the chromatograms show better separation of the eight aminothiols and the analytic time was 20 min. The results demonstrated that it provided an excellent linearity for all analytes over their respective concentration ranges and illustrated excellent precision and plasma recovery as well. Then, the method was applied in the case-control study of patients with diabetes mellitus (DM) and diabetic nephropathy (DN). In conclusion, it is an effective method to quantitate the concentrations of aminothiols in the human plasma. SAH and SAM were suggested as better potential biomarkers; of DM and DN. (C) 2008 Elsevier B.V. All rights reserved.