文献类型：期刊文献

英文题名：Study on Controllable Preparation of Silica Nanoparticles with Multi-sizes and Their Size-dependent Cytotoxicity in Pheochromocytoma Cells and Human Embryonic Kidney Cells

作者：Yuan, Huihui[1,2,3,4,5];Gao, Feng[6];Zhang, Zhigang[1,2];Miao, Lede[1,2];Yu, Ronghua[1,2];Zhao, Hongli[1,2,3,4];Lan, Minbo[1,2,3,4]

机构：[1]E China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China;[4]E China Univ Sci & Technol, Minist Educ, Shanghai 200237, Peoples R China;[5]E China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[6]E China Univ Sci & Technol, Dept Pharmaceut, Sch Pharm, Shanghai 200237, Peoples R China

年份：2010

卷号：56

期号：6

起止页码：632

外文期刊名：JOURNAL OF HEALTH SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000284862900002)】；

基金：This work was supported by the Science and Technology Commission of Shanghai Municipality (STCSM, No. 10dz2220500).

语种：英文

外文关键词：silica nanoparticle; controllable preparation; cytotoxicity; size-dependent

摘要：High-tech products made by nano-materials are tightly connected to people's life. However environmental impacts and health effects of nanoparticles are widely getting attention. In this paper, we focused on the potential size-dependent cytotoxicity of nanomaterials. Silica (SiO(2)) nanoparticles were used as model material. The ion exchange method and modified StOber method were used to controllably prepare monodisperse uniform silica nanoparticles with six types of size (20, 50, 80, 140, 280 and 760 nm). And the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cellular morphology were introduced to evaluate the effect of the six sizes SiO(2) nanoparticles on pheochromocytoma (PC12) and human embryonic kidney (HEK293) cells viability. The results indicated that nanosized-SiO(2)) (20, 50 and 80 nm) caused more cell damage when compared with microsized-SiO(2) (140, 280 and 800 nm). IC(50) of 24 hr exposure in PC12 cells were 118.2 +/- 7.3, 320.4 +/- 9.8, and 380.7 +/- 10.5 mu g/ml, and in HEK293 cells were 80.2 +/- 6.4, 140.3 +/- 9.6 and 309.2 +/- 11.3 mu g/ml for 20, 50- and 80-nm SiO(2) particles, respectively. Additionally, the SiO(2) particles of the other three sizes over 80-nm had little influence on the cell viability at the concentrations below 2000 mu g/ml for two cultured cells. The results suggested that the exposure of SiO(2) nanoparticles with different sizes leaded to cellular morphological modifications and apoptosis in a size-dependent manner. Further studies on the molecular mechanisms of apoptosis and the toxicity in vivo are necessary.