文献类型：期刊文献

英文题名：Determination of aristolochic acids in rat serum by high performance liquid chromatography-Q-TOF tandem mass spectrometry

作者：Gu, Jiangping[1];Zhang, Pinjie[1];Zhang, Feifang[1];Shen, HeQing[2];Yang, Bingcheng[1];Liang, Xinmiao[1];Chu, Xiaogang[3]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Inst Urban Environm, Xiamen 361021, Peoples R China;[3]Chinese Acad Inspect & Quarantine, Inst Food Safety, Beijing 100123, Peoples R China

年份：2013

卷号：5

期号：3

起止页码：718

外文期刊名：ANALYTICAL METHODS

收录：;EI(收录号：20130415939595);WOS:【SCI-EXPANDED(收录号:WOS:000313662800021)】；

基金：This work was financially supported by the Ministry of Science and Technology of China (2011BAK10B04) and the National Natural Science Foundation of China (no. 21075038, 21177040).

语种：英文

外文关键词：Resins - Ascorbic acid - Body fluids - Phase separation - Column chromatography - Inductively coupled plasma - Mass spectrometry - Rats

摘要：Four aristolochic acids (AAs) including AA-I, AA-II, AA-IIIa and AA-IVa in rat serum were simultaneously determined by HPLC-Q-TOF-MS. A new solid phase extraction (SPE) resin with reversed-phase/strong anion exchange mixed groups (C18-SAX) was used to effectively purify and enrich the analytes, which demonstrated much better performance in comparison with common C18-SPE resin in terms of noise level and recovery. The chromatographic separation was performed on a C18 column under isocratic elution, and the effluent was analyzed by TOF-MS-MS in the mode of positive multiple reaction. Naproxen was used as the internal standard (IS). The limit of detection was 3, 6, 0.09 and 1.5 ng mL(-1) for AA-I, AA-II, AA-IIIa and AA-IVa, respectively. Intra-and inter-day precisions measured as relative standard deviation of retention time were less than 1.1% and 1.5%, respectively.