文献类型：期刊文献

英文题名：Synthesis of molecularly imprinted polymer sorbents and application for the determination of aminoglycosides antibiotics in honey

作者：Ji, Shunli[1];Zhang, Feifang[1];Luo, Xi[1];Yang, Bingcheng[1];Jin, Gaowa[2];Yan, Jingyu[2];Liang, Xinmiao[1,2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

年份：2013

卷号：1313

起止页码：113

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20134016810647);WOS:【SCI-EXPANDED(收录号:WOS:000325673200010)】；

基金：The study was financially supported by National Natural Science Foundation of China (no. 21177040, 21075038) and National Major Science Instrument Project of the Ministry of Science and Technology of China (2012YQ090229). It was partly supported by Open Foundation of Key Laboratory of Industrial Ecology and Environmental Engineering.

语种：英文

外文关键词：Molecularly imprinted polymer; HILIC-MS/MS; Aminoglycoside antibiotics; Solid phase extraction; Honey samples

摘要：Aminoglycoside antibiotic (AA)-selective molecularly imprinted polymer (MIP) sorbent was synthesized by polymerization of methacrylic acid and ethylene glycol dimethacrylate in the presence of streptomycin as template molecule. The MIP sorbent was in detail characterized and its performance was evaluated by selecting four model AAs including streptomycin, gentamicin, spectinomycin and dihydrostreptomycin. Relative to non-imprinted polymer (NIP), the MIPs exhibited much higher recognizable capacity and specificity to the AAs and negligible adsorption for non-AAs as well. In combination of hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS), the MIP sorbent-based solid phase extraction (SPE) was able to effectively determine the residue of four model AAs in honey samples with recovery ranging from 90% to 110%. The limit of detection (and the limit of quantitation) of streptomycin, gentamicin, spectinomycin and dihydrostreptomycin was 4.5 (15.0), 2.4 (8.0), 6.0 (20.0) and 1.8 (6.0) mu g/kg, respectively. Relative standard deviations of intraday and inter-day assay under three spiked levels were 4.4-12.0% and 6.8-14.6%, respectively. (c) 2013 Elsevier B.V. All rights reserved.