文献类型：期刊文献

英文题名：Isolation, sequencing and characterization of cluster genes involved in the biosynthesis and utilization of the siderophore of marine fish pathogen Vibrio alginolyticus

作者：Wang, Qiyao; Liu, Qin; Ma, Yue; Zhou, Lingyun; Zhang, Yuanxing

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2007

卷号：188

期号：4

起止页码：433

外文期刊名：ARCHIVES OF MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000249522600015)】；

语种：英文

外文关键词：Vibrio alginolyticus; siderophore; biosynthesis; uptake; cluster gene; plasposon

摘要：In fish pathogen Vibrio alginolyticus MVP01, the isolated 11-gene cluster consisted of two divergently transcribed, Fe3+ and ferric uptake regulator (Fur) regulated operons, pvsABCDE and psuA-pvuABCDE, sharing high similarity with that related to siderophore biosynthesis and transportation locus in V. parahaemolyticus. Siderophore biosynthesis or utilization was blocked when pvsA and pvsD of the pvsABCDE operon or pvuA, pvuB and pvuE of the psuA-pvuABCDE operon was single-gene in-frame mutated, demonstrating their essential roles for siderophore biosynthesis or utilization in V. alginolyticus MVP01. Addition of the purified siderophore restored the cell growth in siderophore biosynthesis mutants, but not in siderophore uptake mutants.