文献类型：期刊文献

中文题名：H_2O_2对枯草芽孢杆菌生长代谢以及腺苷发酵的影响

英文题名：Effects of H_2O_2 on the Growth of Bacillus subtilis ATCC 21616 Metabolism and the Accumulation of Adenosine

作者：莫绯[1];毛利斯[1];高淑红[1];陈长华[1]

机构：[1]国家生物反应器工程国家重点实验室,华东理工大学生物工程学院,上海200237

年份：2007

卷号：38

期号：6

起止页码：416

中文期刊名：中国医药工业杂志

外文期刊名：Chinese Journal of Pharmaceuticals

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：过氧化氢;腺苷;枯草芽孢杆菌

外文关键词：H2O2; adenosine; Bacillus subtilis

摘要：通过摇瓶发酵试验研究H2O2对枯草芽孢杆菌ATCC21616生长代谢及产苷的影响。结果表明:添加时间、浓度以及两者的交互作用显著影响腺苷的积累,在12h添加20mg/L的H2O2最利于腺苷的合成,产苷量由7.03g/L增至7.79g/L。同时也研究了H2O2添加对代谢过程中糖耗及pH的影响。
The effects of H2O2 on the growth, metabolism and the accumulation of adenosine of Bacillus subtilis ATCC 21616 were investigated. The results showed that addition time, concentration and interaction between them could significantly affect the accumulation of adenosine. The best production of adenosine was observed when 20mg/L H2O2 was added to the culture medium at 12h after inoculation. Under these conditions, the adenosine yield was increased from 7.03g/L to 7.79g/L. Changes in sugar uptake and pH during the fermentation were also studied.