文献类型：期刊文献

英文题名：Raman imaging diagnosis of the early stage differentiation of mouse embryonic stem cell (mESC)

作者：Dou, Xiaoming[1,2];Zhao, Yubin[1];Li, Mingda[1];Chen, Qinmiao[1];Yamaguchi, Yoshinori[1,2]

机构：[1]East China Univ Sci & Technol, Sch Sci, Inst Photon & Biomed, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Osaka Univ, Grad Sch Engn, Dept Appl Phys, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan

年份：2020

卷号：224

外文期刊名：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

收录：;EI(收录号：20193107265619);WOS:【SCI-EXPANDED(收录号:WOS:000492371400068)】；

基金：This work was supported by the Research Start-up Fund Project of East China University of Science and Technology (No. YK0142119).

语种：英文

外文关键词：Raman microspectroscopy; Raman imaging; Differentiation; Stem cells; PCA

摘要：Raman microspectroscopy as a non-invasive and label-free technique was applied to diagnose the early stage differentiation of mouse embryonic stem cells. The differentiated and undifferentiated embryonic bodies (EBs) were cultured using handing drop method by the control of Leukemia Inhibitory Factor (LIF). Raman spectra of the periphery cells of differentiated EBs (PrE cells) and those of the interior of undifferentiated EBs (ES cells) were obtained to diagnose the stem cells of different differentiation. It was found from the spectra that the protein content increased as the cells differentiated. Principal component analysis (PCA) was carried out to further analyze the differences between ES cells and PrE cells. The first three principle components contained 98.19% from the total variance. Characteristic bands of ES and PrE cells were chosen to acquire Raman images of two cells according to the results of PCA. In the Raman images, PrE cells had a clear and bright outline in the peripheral areas while ES cells were difficult to identify, this could be a distinct characteristic to discriminate them. The result of the Raman images was consistent with the biological agreement that the differentiated cells were distributed around the periphery. (C) 2019 Elsevier B.V. All rights reserved.