文献类型：期刊文献

英文题名：Bead-Based Mesofluidic System for Residue Analysis of Chloramphenicol

作者：Zhang, Dong[1];Zuo, Peng[1];Ye, Bang-Ce[1]

机构：[1]E China Univ Sci & Technol, Dept Food Engn & Sci, State Key Lab Bioreactor Engn, Lab Biosystm & Microanal, Shanghai 200237, Peoples R China

年份：2008

卷号：56

期号：21

起止页码：9862

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000260675600022)】；

基金：Received for review July 10, 2008. Revised manuscript received September 2, 2008. Accepted September 2, 2008. This work was financially supported by the National Natural Science Foundation (20627005), the Shanghai Municipal Commission for Science and Technology (06DZ19151 and 07dz19508), the Program for New Century Excellent Talents in University (NCET-07-0287). and the Shanghai Shuguang Program (06SG32).

语种：英文

外文关键词：Mesofluidic; immunoassay; glass bead; chloramphenicol; residue detection

摘要：A highly selective and sensitive mesofluidic immunoassay system based on competitive immunoassay in polydimethylsiloxane (PDMS) channels was developed. This immunoassay system was successfully applied to quantificationally detect chloramphenicol (CAP) in animal foods. The glass beads (empty set 250 mu m) were. amino-silane modified, covalently precoated with chloramphenicol succinate, and then infused into the microchannels (empty set 300 mu m); the CAP molecules of samples or standards in flow solution competed for CAP antibody with the CAP immobilized on the beads. The CAP antigen-antibody complex anchored on the beads was probed by Cy5-labeled secondary antibody, and the fluorescence intensities of beads were employed to determine the concentration of CAP. In this system, the detection limit of CAP is 0.008 mu g/L. The method reveals good recovery rates from 90 to 108% and coefficients of variance (CV) from 4.72 to 6.52%. The experimental results demonstrate that the bead-based mesofluidic system has high sensitivity and excellent performance. Indeed, this system can readily be operated automatically and expanded for multicomponent analysis. It is therefore an attractive alternative to conventional immunoassays in routine supervised domain application for contamination in foods or the environment.