文献类型：期刊文献

英文题名：In situ formation of a biomimetic lipid membrane triggered by an aggregation-enhanced photoligation chemistry

作者：Zhou, Yaowu[2];Yang, Huiting[2];Wang, Chenxi[2];Xue, Yuan[2];Wang, Xuebin[2];Bao, Chunyan[1,2];Zhu, Linyong[1,2]

机构：[1]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res C, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Key Lab Adv Mat & Joint Int Res Lab Precis Chem &, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2021

卷号：12

期号：10

起止页码：3627

外文期刊名：CHEMICAL SCIENCE

收录：;EI(收录号：20211310128491);WOS:【SCI-EXPANDED(收录号:WOS:000630166700021)】；

基金：This work is supported by the National Key Research and Development Project (2019YFA0110500) and NSFC (Grant No. 21472044). The authors thank Research Centre of Analysis and Test of East China University of Science and Technology for the help on the characterizations.

语种：英文

外文关键词：Biomimetics - Membranes - Liposomes - Phospholipids

摘要：Nature or synthetic systems that can self-assemble into biomimetic membranes and form compartments in aqueous solution have received extensive attention. However, these systems often have the problems of requiring complex processes or lacking of control in simulating lipid synthesis and membrane formation of cells. This paper demonstrates a conceptually new strategy that uses a photoligation chemistry to convert nonmembrane molecules to yield liposomes. Lysosphingomyelin (Lyso) and 2-nitrobenzyl alcohol derivatives (NBs) are used as precursors and the amphiphilic character of Lyso promotes the formation of mixed aggregates with NBs, bringing the lipid precursors into close proximity. Light irradiation triggers the conversion of NBs into reactive aldehyde intermediates, and the preassembly facilitates the efficient and specific ligation between aldehyde and Lyso amine over other biomolecules, thereby accelerating the synthesis of phospholipids and forming membrane compartments similar to natural lipids. The light-controllable transformation represents the use of an external energy stimulus to form a biomimetic phospholipid membrane, which has a wide range of applications in medicinal chemistry, synthetic biological and abiogenesis.