文献类型：期刊文献

英文题名：Prevalence and characterization of Cryptosporidium spp. in dairy cattle in Nile River delta provinces, Egypt

作者：Amer, Said[1,2];Zidan, Shereif[3];Adamu, Haileeyesus[1,4];Ye, Jianbin[1,5];Roellig, Dawn[1];Xiao, Lihua[1];Feng, Yaoyu[5]

机构：[1]Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA USA;[2]Kafr El Sheikh Univ, Fac Sci, Dept Zool, Kafr Al Sheikh 33516, Egypt;[3]Menoufia Univ, Sadat Branch, Fac Vet Med, Dept Anim Hyg & Zoonoses, Al Menoufia, Egypt;[4]Univ Addis Ababa, Addis Ababa, Ethiopia;[5]E China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China

年份：2013

卷号：135

期号：3

起止页码：518

外文期刊名：EXPERIMENTAL PARASITOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000327283500007)】；

基金：This research was supported by the Arab Fund for Economic & Social Development (Zamalat Program), National Natural Science Foundation of China (Project Nos. 31229005 and 31110103901), and Centers for Disease Control and Prevention.

语种：英文

外文关键词：Cryptosporidium; Cryptosporidiosis; Genotype; Subtype; Cattle; Zoonosis; Molecular epidemiology; Egypt

摘要：Molecular characterizations of Cryptosporidium spp. in dairy cattle in industrialized nations have mostly shown a dominance of Cryptosporidium parvum, especially its Ha subtypes in pre-weaned calves. Few studies, however, have been conducted on the distribution of Cryptosporidium species and C parvum subtypes in various age groups of dairy cattle in developing countries. In this study, we examined the prevalence and molecular characteristics of Cryptosporidium in dairy cattle in four Nile River delta provinces in Egypt. Modified Ziehl-Neelsen acid-fast microscopy was used to screen for Cryptosporidium oocysts in 1974 fecal specimens from animals of different ages on 12 farms. Positive fecal specimens were identified from all studied farms with an overall prevalence of 13.6%. By age group, the infection rates were 12.5% in pre-weaned calves, 10.4% in post-weaned calves, 22.1% in heifers, and 10.7% in adults. PCR-RFLP and DNA sequence analyses of microscopy-positive fecal specimens revealed the presence of four major Cryptosporidium species. In pre-weaned calves, C parvum was most common (30/69 or 43.5%), but Cryptosporidium ryanae (13/69 or 18.8%), Cryptosporidium bovis (7/69 or 10.2%), and Cryptosporidium andersoni (7/69 or 10.2%) were also present at much higher frequencies seen in most industrialized nations. Mixed infections were seen in 12/69 (17.4%) of genotyped specimens. In contrast, C andersoni was the dominant species (193/195 or 99.0%) in post-weaned calves and older animals. Subtyping of C parvum based on sequence analysis of the 60 kDa glycoprotein gene showed the presence of subtypes IIdA20G1 in nine specimens, HaA15G1R1 in 27 specimens, and a rare subtype IIaA14G1R1r1b in one specimen. The common occurrence of non-C parvum species and lid subtypes in pre-weaned calves is a distinct feature of cryptosporidiosis transmission in dairy cattle in Egypt. The finding of the same two dominant Ha and lid C parvum subtypes recently found in humans in Egypt suggests calves can be potential reservoirs of zoonotic cryptosporidiosis. Published by Elsevier Inc.