文献类型：期刊文献

中文题名：畜禽养殖场污水处理系统中诺氟沙星抗性菌的筛选及其抗性基因分析

英文题名：Isolation of Norfloxacin Resistant Bacteria and Analysis of Its Antibiotic Resistance Genes From the Wastewater Treatment System of a Livestock Farm

作者：赵翰斌[1];陈鑫[1];武广哲[1];周纯淳[1];刘莉莉[1]

机构：[1]华东理工大学资源与环境工程学院/国家环境保护化工过程环境风险评价与控制重点实验室

年份：2020

卷号：36

期号：2

起止页码：250

中文期刊名：生态与农村环境学报

外文期刊名：Journal of Ecology and Rural Environment

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家水体污染控制与治理科技重大专项(2017ZX07202006)

语种：中文

中文关键词：畜禽养殖场;氟喹诺酮类抗生素;鞘氨醇单胞菌;抗生素抗性基因

外文关键词：livestock farm;fluoroquinolones;Sphingomonas sp.;antibiotic resistance genes

摘要：畜禽养殖场污水处理系统中抗生素抗性菌、抗性基因的富集和扩散对生态环境和人类健康具有潜在风险,因此有必要对畜禽养殖污水生物处理系统中的抗性菌、抗性基因的种类和遗传特性等进行研究。笔者从江苏某肉鹅养殖场污水处理系统的二沉池污泥样品中分离得到一株诺氟沙星抗性菌TY-1,根据其形态、生理生化特征及16S rRNA基因鉴定结果,该菌株属于鞘氨醇单胞菌属(Sphingomonas)。对鞘氨醇单胞菌属的全基因组分析表明,菌株TY-1含多种抗生素抗性蛋白,包括腺苷三磷酸结合盒转运蛋白(ABC transporter)、主要易化子超家族转运蛋白(MFS transporter)和细胞色素P450脱氢酶,与鞘氨醇单胞菌属的对应功能酶具有很高的同源性。对比鞘氨醇单胞菌属抗生素抗性基因的基因簇发现,这些抗性基因在鞘氨醇单胞菌属中普遍存在,表明其在该菌属中有一定的保守性。研究结果可为畜禽养殖场污水处理系统中含抗生素抗性基因的致病微生物的生态风险评估等相关研究提供参考。
The accumulation and diffusion of antibiotic resistant bacteria and resistance genes in wastewater treatment sys tems of livestock farm poses potential hazards to ecological environment and human health.Therefore,it is necessary to in vestigate the types of resistant bacteria,resistance genes and genetic characteristics in the livestock wastewater treatment systems.In this study,a norfloxacin resistant strain TY 1 was isolated from the sludge,which was collected from the sec ondary sedimentation tank of a meat goose farm in Jiangsu Province.The strain TY 1 was identified as Sphingomonas sp.,according to the results of morphological,physiological and biochemical characteristics,and 16S rRNA gene sequence a nalysis.Based on the whole genome annotation of Sphingomonas sp.,it was found that strain TY 1 had multiple antibiotic resistance genes(ARGs),including ABC transporter,MFS transporter and cytochrome P450 dehydrogenase.According to the results of gene cluster analysis,these ARGs were ubiquitous in genus of Sphingomonas.This study can provide a useful reference for the ecological risk assessment of pathogenic microorganisms containing antibiotic resistance genes in the wastewater treatment systems of livestock farm.