文献类型：期刊文献

英文题名：Simultaneous quantification of 11 pivotal metabolites in neural tube defects by HPLC-electrospray tandem mass spectrometry

作者：Wang, Yong[3];Zhang, Hong-Yang[3];Liang, Qiong-Lin[1];Yang, Hui-Hua[1];Wang, Yi-Ming[1];Liu, Qing-Fei[4];Hu, Ping[5];Zheng, Xiao-Ying[2];Song, Xin-Ming[2];Chen, Gong[2];Zhang, Ting[6];Wu, Jian-Xin[6];Luo, Guo-An[1,3]

机构：[1]Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Bioorgan Phosphorous Chem & Chem Biol, Beijing 100084, Peoples R China;[2]Peking Univ, Inst Populat Res, WHO Collaborating Ctr Res Reprod Hlth & Populat S, Beijing 100871, Peoples R China;[3]E China Univ Sci & Technol, Sch Pharmaceut, Shanghai 200237, Peoples R China;[4]Tsinghua Univ, Sch Med, Beijing, Peoples R China;[5]E China Univ Sci & Technol, Coll Chem & Mol Engn, Shanghai 200237, Peoples R China;[6]Capital Inst Pediat, Beijing, Peoples R China

年份：2008

卷号：863

期号：1

起止页码：94

外文期刊名：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

收录：;EI(收录号：20080711098959);WOS:【SCI-EXPANDED(收录号:WOS:000253594100012)】；

语种：英文

外文关键词：neural tube defects; one-carbon metabolism; tandem mass spectrometry; folate; homocysteine

摘要：One-carbon metabolism that involves folate metabolism and homocysteine metabolism plays a powerful role in embryonic development. Any impairment to this metabolism during the neurulation process would trigger the occurrence of neural tube defects (NTDs). The great importance of one-carbon metabolism necessitates the establishment of methodology to determine the relative compounds involved in the metabolic cycles. We have developed a sensitive method for measurement of 11 pivotal compounds by using high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS/MS) in sera of pregnant women. Use of an aqueous chromatography column increased retention time and separation of the polar compounds in the system, resulting in fewer co-elution and interference from the other compounds that can lead to ion suppression. Calibration curves suitable for the analysis of maternal serum were linear (r(2) > 0.997) with limits of detection from 0.05 to 1 ng/mL. Intra-day coefficients of variation (CVs) and inter-day CVs were both lower than 11%. With the developed method, 96 serum samples including 46 cases and 50 controls were analyzed. The established method provided a reliable method for quantifying most of the compounds involved in the one-carbon metabolism simultaneously, thus made it possible to elucidate NTDs with multiple factors instead of one single and provided a solid foundation for the diagnosis and prevention of NTDs as well as some other one-carbon metabolism related diseases. (C) 2008 Published by Elsevier B.V.