文献类型：期刊文献

英文题名：A novel strategy of gifting antibacterial activity to the waste from food processing: Probiotic fermentation combining with enzymatic hydrolysis of rice wine residue

作者：Zhang, Yuzhi[1];Luo, Jinnan[1];Du, Lei[1];Gao, Bei[1];Zhao, Li[1];Xie, Jingli[1,2]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, Dept Food Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg SCICB, Shanghai 200237, Peoples R China

年份：2025

卷号：69

外文期刊名：FOOD BIOSCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001504668300001)】；

基金：This work was supported by the National Key Research and Devel-opment Program of China (No. 2020YFA0907800) . The authors thank Research Center of Analysis and Test (East China University of Science and Technology, China) and Instrumental Analysis Center (Shanghai Jiao Tong University, China) for their excellent help.

语种：英文

外文关键词：Rice wine residue; Lactiplantibacillus plantarum X7022; Staphylococcus aureus; Antimicrobial peptides

摘要：This study proposed a strategy combining enzymatic hydrolysis and probiotic fermentation for sustainable utilization of the residue from the processing of Chongming Baijiu, a kind of traditional glutinous rice wine in China. The residue is featured with abundant hydrophobic amino acids content, thereby taken advantage for the production of antimicrobial peptides (AMPs). After the comparison of the hydrolysis with pepsin and alkaline protease, the hydrolysate of alkaline protease with higher hydrolysis degree and more plenty of peptides less than 1 kDa was chosen as the substance for the further fermentation by Lactiplantibacillus plantarum X7022 possessing 84 types of peptidases or proteases among its genome. The product (RRPHF) can effectively inhibit the growth of Staphylococcus aureus by penetrating the biofilm, and then inhibiting the cell survivability as well as damaging the cellular integrity. There are 646 peptides identified from the RRPHF through Nano HPLC MS/MS, with 75 pieces of high-scoring AMPs predicted. Moreover, enzyme cleavage site analysis and peptide molecular weight determination confirmed that the peptidases or proteases of L. plantarum X7022 could effectively produce AMPs rich in hydrophobic amino acids. The results demonstrated that combining enzymatic hydrolysis with probiotic fermentation is a prospect approach for the AMPs yielding, offering a feasibly substantial pathway for the high-value application of byproduct in food industry.