文献类型：期刊文献

英文题名：Dysregulated haemolysin promotes bacterial outer membrane vesicles-induced pyroptotic-like cell death in zebrafish

作者：Wen, Ying[1];Chen, Shouwen[1];Jiang, Zhiwei[1];Wang, Zhuang[1];Tan, Jinchao[1];Hu, Tianjian[1];Wang, Qiyao[1,2,3,4];Zhou, Xiangshan[1];Zhang, Yuanxing[1,3,4];Liu, Qin[1,2,3,4];Yang, Dahai[1,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai, Peoples R China;[4]Shanghai Engn Res Ctr Marine Cultured Anim Vaccin, Shanghai, Peoples R China

年份：2019

卷号：21

期号：6

外文期刊名：CELLULAR MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000468783500002)】；

基金：National Natural Science Foundation of China, Grant/Award Numbers: 31430090 and 31622059; Shanghai Chenguang Program, Grant/Award Number: 16CG33; Young Elite Scientists Sponsorship Program, Grant/Award Number: 2016QNRC001; Fundamental Research Funds for the Central Universities, Grant/Award Numbers: 222201718004 and 222201717019

语种：英文

外文关键词：bacterial outer membrane vesicles; Edwardsiella piscicida; haemolysin; pyroptotic-like cell death; zebrafish intestinal infection

摘要：Inflammasomes are important innate immune components in mammals. However, the bacterial factors modulating inflammasome activation in fish, and the mechanisms by which they alter fish immune defences, remain to be investigated. In this work, a mutant of the fish pathogen Edwardsiella piscicida (E. piscicida), called 0909I, was shown to overexpress haemolysin, which could induce a robust pyroptotic-like cell death dependent on caspase-5-like activity during infection in fish nonphagocyte cells. E. piscicida haemolysin was found to mainly associate with bacterial outer membrane vesicles (OMVs), which were internalised into the fish cells via a dynamin-dependent endocytosis and induced pyroptotic-like cell death. Importantly, bacterial immersion infection of both larvae and adult zebrafish suggested that dysregulated expression of haemolysin alerts the innate immune system and induces intestinal inflammation to restrict bacterial colonisation in vivo. Taken together, these results suggest a critical role of zebrafish innate immunity in monitoring invaded pathogens via detecting the bacterial haemolysin-associated OMVs and initiating pyroptotic-like cell death. These new additions to the understanding of haemolysin-mediated pathogenesis in vivo provide evidence for the existence of noncanonical inflammasome signalling in lower vertebrates.