文献类型：期刊文献

英文题名：NPtagM: A Tailoring Enzyme Genome Mining Toolkit and Its Application in Terpenoid P450s from Phytopathogenic Fungi

作者：Cong, Zhanren[1];Mukoma, Njeru Joe[1];Yin, Qiang[1];Zhu, Bin[1,2,3];She, Lingwei[4];Hsiang, Tom[5];Zhang, Lixin[1];Jiang, Lan[6];Liu, Xueting[1]

机构：[1]East China Univ Sci Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Lab Pharmaceut Crystal Engn & Technol, Shanghai 200237, Peoples R China;[4]Beijing Inst Technol, Sch Life Sci, Key Lab Mol Med & Biotherapy, Beijing 100081, Peoples R China;[5]Univ Guelph, Sch Environm Sci, Guelph, ON N1G2W1, Canada;[6]Nanjing Med Univ, Childrens Hosp, Dept Cardiothorac Surg, Nanjing 210093, Peoples R China

年份：2024

卷号：72

期号：49

起止页码：27225

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20244917491393);WOS:【SCI-EXPANDED(收录号:WOS:001368399800001)】；

基金：This work was supported by the Science and Technology Commission of Shanghai Municipality (21NL2600100), National Key Research and Development Program of China (2020YFA090032 and 2019YFA0906200), the National Natural Science Foundation of China (21977029 and 21907031), and the Open Project Funding of the State Key Laboratory of Bioreactor Engineering, The 111 Project (B18022). Furthermore, we would like to acknowledge the Natural Science and Engineering Research Council of Canada for funding the genome sequencing and assembly of fungal genomes under the supervision of Prof. T. Hsiang.

语种：英文

外文关键词：NPtagM; tailoring enzyme genome mining; terpenoids; cytochrome P450; phytopathogenic fungi

摘要：Terpenoids derived from phytopathogenic fungi are major participants in interactions among microorganisms, plants, and animals. The modifications catalyzed by cytochrome P450s significantly influence the structural and bioactivity diversity of the terpenoids. To conduct genome mining of P450s in pathogenic fungi, in this study, we developed a new software called Natural Products Tailoring Enzymes Genome Mining (NPtagM). By optimizing the workflow and gene prediction software, NPtagM demonstrated a 3-fold increase in the number of predicted P450s and an 8-fold reduction in runtime compared to antiSMASH. We then used it to extract 1189 dereplicated terpenoid P450s from our in-house fungal genomes. Using a sequence similarity network analysis, we identified a family that potentially produced eremophilane-type sesquiterpenoids. The heterologous expression in Aspergillus oryzae resulted in the production of two new and four known eremophilanes. Our results highlight the potential of NPtagM in genome mining for tailoring enzymes from phytopathogenic fungi.