文献类型：期刊文献

英文题名：Metabolic Engineering of Saccharomyces cerevisiae for High-Level Production of Salidroside from Glucose

作者：Jiang, Jingjie[1];Yin, Hua[2,3];Wang, Shuai[2,4];Zhuang, Yibin[2,3];Liu, Shaowei[1];Liu, Tao[2,3];Ma, Yanhe[2,3]

机构：[1]East China Univ Sci & Technol, Coll Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China;[3]Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China;[4]Tianjin Univ Sci & Technol, Coll Biotechnol, Natl & Local United Engn Lab Metab Control Fermen, Tianjin 300457, Peoples R China

年份：2018

卷号：66

期号：17

起止页码：4431

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20181905141372);WOS:【SCI-EXPANDED(收录号:WOS:000431600200016)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (nos. 31770104, 21302214, and 31400026) and the Biological Resources Service Plan of CAS (no. ZSTH-023).

语种：英文

外文关键词：Saccharomyces cerevisiae; salidroside; tyrosol; glucosyltransferase

摘要：Salidroside is an important plant-derived aromatic compound with diverse biological properties. Because of inadequate natural resources, the supply of salidroside is currently limited. In this work, we engineered the production of salidroside in yeast. First, the aromatic aldehyde synthase (AAS) from Petroselinum crispum was overexpressed in Saccharomyces cerevisiae when combined with endogenous Ehrlich pathway to produce tyrosol from tyrosine. Glucosyltransferases from different resources were tested for ideal production of salidroside in the yeast. Metabolic flux was enhanced toward tyrosine biosynthesis by overexpressing pathway genes and eliminating feedback inhibition. The pathway genes were integrated into yeast chromosome, leading to a recombinant strain that produced 239.5 mg/L salidroside and 965.4 mg/L tyrosol. The production of salidroside and tyrosol reached up to 732.5 and 1394.6 mg/L, respectively, by fed-batch fermentation. Our work provides an alternative way for industrial large-scale production of salidroside and tyrosol from S. cerevisiae.