文献类型：期刊文献

英文题名：An effective and simplified pH-stat control strategy for the industrial fermentation of vitamin B₁₂ by Pseudomonas denitrificans

作者：Li, Kun-Tai[1];Liu, Dong-Hong[1];Chu, Ju[1];Wang, Yong-Hong[1];Zhuang, Ying-Ping[1];Zhang, Si-Liang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2008

卷号：31

期号：6

起止页码：605

外文期刊名：BIOPROCESS AND BIOSYSTEMS ENGINEERING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000258833600011)】；

基金：This work was supported by National Basic Research Program (973 Program, No. 2007CB714303) and Shanghai Leading Academic Discipline Project (B505). We are grateful to Shijiazhuang Pharma. Group Hua Rong Pharmaceutical Co. Ltd for their support in this work.

语种：英文

外文关键词：pH-stat; Pseudomonas denitrificans; vitamin B12

摘要：In order to improve the productivity of vitamin B(12) by Pseudomonas denitrificans carried out in a 120-m(3) fermenter, the effect of pH on vitamin B(12) biosynthesis was investigated. Results obtained from shake flask experiments showed that the feeding of carbon source (beet molasses or glucose) and methyl-group donor (betaine or choline chloride) significantly influenced the pH and the biosynthesis of vitamin B(12). In contrast to beet molasses or choline chloride, using glucose as a feed medium and betaine as a methyl-group donor, pH could be maintained at a stable range. As a result, higher vitamin B(12) production was achieved. Accordingly, an effective and simplified pH-stat control strategy was established for the fermentation of vitamin B(12) in a 120-m(3) industrial fermenter. When the new pH control strategy was applied, pH was stably kept in the range of 7.15-7.30 during fermentation. Thus, 214.3 mu g/mL of vitamin B(12) was achieved.