文献类型：期刊文献

英文题名：An injectable scaffold based on crosslinked hyaluronic acid gel for tissue regeneration

作者：Yang, Rui[1];Tan, Linhua[1];Cen, Lian[1];Zhang, Zhibing[2]

机构：[1]E China Univ Sci & Technol, Dept Prod Engn, Sch Chem Engn, Shanghai Key Lab Multiphase Mat Chem Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]Univ Birmingham, Sch Chem Engn, Birmingham B15 2TT, W Midlands, England

年份：2016

卷号：6

期号：20

起止页码：16838

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20160801962763);WOS:【SCI-EXPANDED(收录号:WOS:000370710600094)】；

基金：This study is financially supported by "the Fundamental Research Funds for the Central Universities", National "973" Project Foundation (2010CB944804) and National Nature Science Foundation (81471855). The authors confirm that there are no known confiicts of interest associated with this publication.

语种：英文

外文关键词：Cartilage - Hydrogels - Organic acids - Scaffolds (biology) - Histology - Viscoelasticity - Tissue regeneration

摘要：Injectable scaffolds have great potential in specialised applications in regenerative medicine. In this study, hyaluronic acid hydrogels (HAGs) were prepared by crosslinking hyaluronic acid (HA) with 1,4-butanediol diglycidyl ether (BDDE). Applications of HAG as an injectable scaffold for regenerating functional tissues were proposed by matching its viscoelastic properties with those of biological tissues. The effect of BDDE concentration on different properties of HAGs was explored. Swelling properties, cross-sectional morphology, and BDDE residues of the resulting gels were investigated. Rheological properties of different HAGs were measured by monitoring their storage modulus (G') and loss modulus (G '') and compared with those of biological tissues. It was shown that HAGs (BDDE from 0.4 vol% to 1.0 vol%) possess great water absorbing capability with swelling ratios ranging from 99.7 to 78.9. The higher the concentration of the crosslinker used, the more rigid the resulting hydrogel, subsequently the lower the swelling ratio would be and the higher the G' and G '' values as well. Similar viscoelastic behaviors were found between HAGs and biological tissues, such as epidermis, dermis, articular cartilage and tooth germ. SEM revealed that HAG obtained at 0.4 vol% BDDE had pore diameters ranging from a few microns to around 100 mm with a high degree of interconnectivity. The feasibility of this HAG, as an injectable scaffold, to regenerate cartilage and dentin-pulp complex was then demonstrated using a preliminary subcutaneous microenvironment. The current study could be a reference to take account of how a crosslinked HA gel should be chosen for specific tissue regeneration.