文献类型：期刊文献

英文题名：In Vitro Investigation of 2′,4′-Dihydroxy-6′-methoxy-3′,5′-dimethylchalcone for Glycemic Control

作者：Hu, Ying-Chun[1,2];Luo, Ying-Di[1];Li, Lin[2];Joshi, Manoj Kumar[3];Lu, Yan-Hua[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Unilever R&D Shanghai, Shanghai 200335, Peoples R China;[3]Unilever R&D Bangalore, Bangalore 560066, Karnataka, India

年份：2012

卷号：60

期号：42

起止页码：10683

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20124415615884);WOS:【SCI-EXPANDED(收录号:WOS:000310104000038)】；

基金：This work was supported by "the Fundamental Research Funds for the Central Universities" and partially supported by Shanghai Leading Academic Discipline Project (13505), the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204).

语种：英文

外文关键词：2 ',4 '-dihydroxy-6 '-methoxy-3 ',5 '-dimethylchalcone; pancreatic alpha-amylase; intestinal alpha-glucosidase; intestinal glucose transporters; oxidative stress; insulin secretion

摘要：2',4'-Dihydroxy-6'-methoxy-3',5'-dimethylchalcone (DMC), a compound isolated and purified from the dried flower buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry (Myrtaceae), was investigated for its glucose control benefits using in vitro methods. DMC showed strong noncompetitive (IC50 of 43 mu M) inhibition of pancreatic alpha-amylase; it was, however, ineffective against intestinal alpha-glucosidase. In addition, DMC exhibited remarkable glucose transport inhibition effects in both simulated fasting and fed states in Caco-2 cell monolayers (P < 0.05). Besides, exposure of MIN6 cells to 250 mu M H2O2 for 1 h caused a significant viability loss and insulin secretion reduction. Pretreatment of MIN6 cells with DMC for 2 h protected against the H2O2-induced decrease in glucose-stimulated insulin secretion in a dose-dependent manner and also enhanced the impaired basal insulin secretion. Such effects highlight the therapeutic potential of DMC in the management of hyperglycemia.