文献类型：期刊文献

英文题名：Supercritical fluid-based method for selective extraction and analysis of indole alkaloids from Uncaria rhynchophylla

作者：Fu, Qing[1];Dong, Wenwen[1];Ge, Dandan[1];Ke, Yanxiong[1];Jin, Yu[1]

机构：[1]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Minist Educ, Shanghai 200237, Peoples R China

年份：2023

卷号：1710

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20233914810527);WOS:【SCI-EXPANDED(收录号:WOS:001088532000001)】；

语种：英文

外文关键词：Supercritical fluid extraction (SFE); Supercritical fluid chromatography/tandem; mass spectrometry (SFC-MS/MS); Uncaria rhynchophylla; Alkaloids; Selective extraction

摘要：The development of an approach based on simultaneous supercritical fluid extraction-sample cleanup, followed by supercritical fluid chromatography/tandem mass spectrometry (SFE-SFC-MS/MS) was as a tool for the extraction, separation and characterization of indole alkaloids of Uncaria rhynchophylla. A two-step SFE method was designed. A mixture of the U. rhynchophylla sample and an adsorbent named C18SCX with the ratio of 1:1 (w/w) was placed into an extraction cell. The extraction temperature was 40 degrees C and the pressure was 25 Mpa. In the first step, 10 % EtOH as the co-solvent was used to extract for 60 min, which was considered as a cleanup process to remove non-alkaloid components. In the second step, 0.1 % DEA was added to 10 % EtOH and it extracted for 60 min to obtain the desired extract. By introducing an additional adsorbent, the specificity of SFE towards alkaloids was greatly improved. An SFC-MS/MS method was then utilized for analysis of the SFE extract. Using 2-EP as stationary phase with the gradient elution of 0-10 min, 5-25 % EtOH (+0.05 % DEA) in CO2, column temperature 40 degrees C, and back pressure 13.8 Mpa, 10 peaks were separated within 8 min. Further MS/MS analysis confirmed that nine of the 10 peaks in the SFE extract were indole alkaloids. This study developed a supercritical fluid-based method specifically towards extraction and analysis of alkaloids, which is helpful to the study of alkaline compounds in complex samples.