文献类型：期刊文献

英文题名：Redirecting carbon flux in Torulopsis glabrata from pyruvate to α-ketoglutaric acid by changing metabolic co-factors

作者：Huang, H.-J.[1]; Liu, L.-M.[1]; Li, Y.[1,2]; Du, G.-C.[1]; Chen, J.[1]

机构：[1] Key Laboratory of Industrial Biotechnology, School of Biotechnology, Southern Yangtze University, 214036, Wuxi, China; [2] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China

年份：2006

卷号：28

期号：2

起止页码：95

外文期刊名：Biotechnology Letters

收录：EI(收录号：2005529617168)

语种：英文

外文关键词：Carbon - Carboxylic acids - Metabolism - Nutrition - Vitamins

摘要：The nutrition conditions needed to redirect the carbon flux in Torulopsis glabrata, a pyruvate hyper-production yeast, from pyruvate to α-ketoglutaric acid (KG) were investigated in a stirred fermentor. A minor amount of KG (1.3 gl-1) was produced when NaOH was used to control the pH, while 12 g KG l-1 was produced when CaCO3 was used instead. When thiamine and biotin were included in the medium, 13 g KG l -1 and 68 g pyruvate l-1 were produced after 48 h when glucose was nearly consumed (approximately 5 gl-1). With fermentation continuing for a further 16 h, the concentration of pyruvate decreased to 31 gl-1, and KG increased to 30 gl-1. KG thus accumulated at the expense of pyruvate consumption. ? Springer 2006.