文献类型：期刊文献

英文题名：Ti4+-immobilized chitosan-coated magnetic graphene oxide for the highly selective enrichment of phosphopeptides

作者：Zhao, Yiman[1];Zhang, Lingyi[1];Chu, Zhanying[1];Xiong, Zhichao[2];Zhang, Weibing[1]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China

年份：2017

卷号：9

期号：3

起止页码：443

外文期刊名：ANALYTICAL METHODS

收录：;EI(收录号：20170403276337);WOS:【SCI-EXPANDED(收录号:WOS:000393102400010)】；

基金：We gratefully acknowledge the support of the State Key Program of the National Natural Science of China (21235005), the National Natural Science Foundation of China (No. 21475044), the National Key Technology R&D Program (2015BAK44B00) and the National Key Scientific Instrument and Equipment Development Project (2012YQ120044).

语种：英文

外文关键词：Metal ions - Metals - Titanium compounds - Reusability - Affinity chromatography - Graphene - Molecular biology

摘要：For in-depth analysis of phosphorylated proteomics, the highly sensitive and selective capture of phosphopeptides from intricate biological samples is extremely significant. In this work, a late-model immobilized metal ion affinity chromatography (IMAC) material was fabricated and applied to enriching phosphopeptides from biological samples by abundant Ti4+ ions, which were chelated to crosslinked chitosan (CS) loaded on the surface of magnetic graphene oxide (GO) (denoted as mag GO-CS-Ti4+). The high amount of crosslinked CS endowed the IMAC material with highly hydrophilic properties and abundant active sites for Ti4+ ions on the surface. These characteristics ensure the great performance of mag GO-CS-Ti4+ in the selective enrichment of phosphopeptides from the tryptic digest of beta-casein with high selectivity (phosphopeptides to non-phosphopeptides at a molar ratio of 1 : 400), high sensitivity (0.5 fmol), large enrichment capacity (66.6 mg g(-1)), prominent phosphopeptide recovery (93.11%), and excellent reusability. What's more, mag GO-CS-Ti4+ was applied to capturing phosphopeptides from real biological samples, human serum from both healthy people and patients and nonfat milk. The results successfully manifested that the mag GO-CS-Ti4+ could be a great affinity material for the enrichment of low-abundant phosphopeptides from biological samples.