文献类型：期刊文献

中文题名：高效液相色谱内标法测定15-羟基十五烷酸的含量

英文题名：Determination of 15-Hydrocypentacecoic Acid by HPLC Method

作者：刘源源[1];韩丽丽[1];江菲菲[1];彭阳峰[1]

机构：[1]华东理工大学化工学院,上海200237

年份：2011

卷号：52

期号：11

起止页码：654

中文期刊名：化学世界

外文期刊名：Chemical World

收录：北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：15-羟基十五烷酸;十四酸;高效液相色谱;内标法

外文关键词：15-hydrocypentacecoic acid; myristic acid; HPLC; internal standard

摘要：用液相色谱内标法定量分析15-羟基十五烷酸。样品以C18(4.6×250 mm)为色谱柱,以十四酸为内标物,流动相为甲醇∶乙腈∶水=80∶5∶15(0.1%三氟乙酸),流速:1.2 mL/min,检测波长为210 nm。结果表明在实验范围内,方法的相关性好(r>0.999 0),样品测定的相对标准偏差RSD<1%,加标回收率为100.36%,检出限为7.29×10-4g/(s.L)。
The method of high performance liquid chromatography（HPLC） with internal standard was applied to the quantitative analysis of 15-hydrocypentacecoic acid.C18（4.6×250 mm） was used and myristic acid was the internal standard.The mobile phase was a mixture of methanol,acetonitrile and water（80∶5∶15,0.1% trifluoroacetic acid）,the flow rate was 1.2 mL/min and UV detection was performed at 210 nm.Using this method,the desired relativity（r0.999） and repeatability（RSD1%） were obtained,the average recovery rate was 100.36% and the sensitivity was 7.29×10-4g/（s·L）.