文献类型：期刊文献

英文题名：Carbamate insecticide methomyl confers cytotoxicity through DNA damage induction

作者：Xiang Guanggang[1];Li Diqiu[1];Yuan Jianzhong[2];Guan Jingmin[2];Zhai Huifeng[3];Shi Mingan[2];Tao Liming[1]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China;[3]Yangzhou Univ, Coll Hort & Plant Protect, Dept Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China

年份：2013

卷号：53

起止页码：352

外文期刊名：FOOD AND CHEMICAL TOXICOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000322924700048)】；

基金：We would like to express our cordial gratitude to Prof. Larry Murdock from Department of Entomology, Purdue University and Prof. Qingchun Huang from School of Pharmacy, East China University of Science and Technology, for their remarkable revisions. This work was supported by Grants from the National Key Project for Basic Research (2010CB126100) and the National Natural Science Foundation of China (No. 31071982).

语种：英文

外文关键词：Methomyl; DNA damage; Apoptosis; Alkaline comet assay; gamma H2AX foci; DNA fragmentation

摘要：Carbamate insecticide methomyl could induce genotoxic effects, including micronuclei, chromosome aberrations and sister-chromatid exchanges. However, methomyl induction of cytotoxicity through DNA damage is largely unknown. Here we identify cytotoxicity and potential genotoxicity of methomyl in vitro. We have employed alkaline comet assay, gamma H2AX foci formation and DNA ladder assay to detected DNA damage and apoptosis of Drosophila S2, HeLa and HEK293 cells. The alkaline comet assay was used to evaluate total DNA single strand breaks (SSBs) in the target cells exposed in vitro to sublethal concentrations of methomyl. As expected, methomyl induced significant concentration-dependent increases in DNA damage of target cells compared with the negative control, as measured by increases in tail length (urn), tail DNA (percentage of the comet tail) and tail moment (arbitrary units). In agreement with the comet assay data, the percentage of gamma H2AX positive reaction in HeLa cells also revealed methomyl caused DNA double strand breaks (DSBs) in a time-dependent manner. Moreover, methomyl induced a significant increase of apoptosis in Drosophila 52, HeLa and HEK293 cells in a concentration- and time-dependent manner, as determined by Urea PAGE DNA fragmentation analysis. In conclusion, methomyl is a strongly genotoxic agent that induces cell DNA damage and apoptosis in vitro at these sublethal concentrations. (c) 2012 Elsevier Ltd. All rights reserved.